ArticleNucleic acids research2005
Transformation of isolated mammalian mitochondria by bacterial conjugation.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 138 citations in OpenAlex.
- Clinical outcomes with alternative dosing strategies for piperacillin/tazobactam: a systematic review and meta-analysis.PloS one · 2015Pooled it
- Mapping and editing animal mitochondrial genomes: can we overcome the challenges?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Modifying the Mitochondrial Genome.Cell metabolism · 2016Review
- Transferring isolated mitochondria into tissue culture cells.Nucleic acids research · 2012Article
- Article
- Nonreplicating intracellular bacterial vector for conjugative DNA transfer into mitochondria.Pharmaceutical research · 2012Article
- Article
- Mitochondrial transformation: time for concerted action.EMBO reports · 2011Article
- Re-engineering the mitochondrial genomes in mammalian cells.Anatomy & cell biology · 2010Article
- Selection by drug resistance proteins located in the mitochondria of mammalian cells.Mitochondrion · 2008Article
- Bacterial conjugation in the cytoplasm of mouse cells.Infection and immunity · 2008Article
- Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei.Nucleic acids research · 2008Article
- Interspecies mitochondrial fusion between mouse and human mitochondria is rapid and efficient.Mitochondrion · 2007Article
- Gene therapy of the other genome: the challenges of treating mitochondrial DNA defects.Pharmaceutical research · 2007Review
- High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a method for transferring exogenous DNA molecules into isolated mammalian mitochondria using bacterial conjugation. In general, we accomplish this by (i) inserting an origin of DNA transfer (oriT) sequence into a DNA construct, (ii) transforming the construct into an appropriate Escherichia coli strain and then (iii) introducing the mobilizable DNA into mitochondria through conjugation. We tested this approach by transferring plasmid DNA containing a T7 promoter sequence into mitochondria that we had engineered to contain T7 RNA polymerase. After conjugation between E.coli and mitochondria, we detected robust levels of T7 transcription from the DNA constructs that had been transferred into the mitochondria. This approach for engineering DNA constructs in vitro and subsequent transfer into mitochondria by conjugation offers an attractive experimental system for studying many aspects of vertebrate mitochondrial gene expression and is a potential route for transforming mitochondrial networks within mammalian cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.