ArticleThe Journal of cell biology2005
Nebulin regulates the assembly and lengths of the thin filaments in striated muscle.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 85 citations in OpenAlex.
- Cypher/ZASP drives cardiomyocyte maturation via actin-mediated MRTFA-SRF signalling.Theranostics · 2024Article
- A long-read RNA-seq approach to identify novel transcripts of very large genes.Genome research · 2020Article
- Nebulin: big protein with big responsibilities.Journal of muscle research and cell motility · 2020Review
- Two alternatively-spliced human nebulin isoforms with either exon 143 or exon 144 and their developmental regulation.Scientific reports · 2018Article
- Aberrant developmental titin splicing and dysregulated sarcomere length in Thymosin β4 knockout mice.Journal of molecular and cellular cardiology · 2017Article
- Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.FEBS letters · 2016Article
- Thin filament length in the cardiac sarcomere varies with sarcomere length but is independent of titin and nebulin.Journal of molecular and cellular cardiology · 2016Article
- Comprehensive Transcriptome Analysis of Sex-Biased Expressed Genes Reveals Discrete Biological and Physiological Features of Male and Female Schistosoma japonicum.PLoS neglected tropical diseases · 2016Article
- Nebulin, a multi-functional giant.The Journal of experimental biology · 2016Review
- Investigation of Pathogenic Genes in Chinese sporadic Hypertrophic Cardiomyopathy Patients by Whole Exome Sequencing.Scientific reports · 2015Article
- Cofilin-2 controls actin filament length in muscle sarcomeres.Developmental cell · 2014Article
- The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils.The Journal of cell biology · 2014Article
- Does Nebulin Make Tropomyosin Less Dynamic in Mature Myofibrils in Cross-Striated Myocytes?Journal of cytology & histology · 2014Article
- Identification of Xin-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling.Molecular biology of the cell · 2013Article
- Clenbuterol plus granulocyte colony-stimulating factor regulates stem/progenitor cell mobilization and exerts beneficial effect by increasing neovascularization in rats with heart failure.Journal of cardiac failure · 2013Article
- Mammalian formin Fhod3 plays an essential role in cardiogenesis by organizing myofibrillogenesis.Biology open · 2012Article
- Neb: a zebrafish model of nemaline myopathy due to nebulin mutation.Disease models & mechanisms · 2012Article
- The desmin coil 1B mutation K190A impairs nebulin Z-disc assembly and destabilizes actin thin filaments.Journal of cell science · 2011Article
- Nebulin, a major player in muscle health and disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Review
- The Nebulin family: an actin support group.Trends in cell biology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In many tissues, actin monomers polymerize into actin (thin) filaments of precise lengths. Although the exact mechanisms involved remain unresolved, it is proposed that "molecular rulers" dictate the lengths of the actin filaments. The giant nebulin molecule is a prime candidate for specifying thin filament lengths in striated muscle, but this idea has never been proven. To test this hypothesis, we used RNA interference technology in rat cardiac myocytes. Live cell imaging and triple staining revealed a dramatic elongation of the preexisting thin filaments from their pointed ends upon nebulin knockdown, demonstrating its role in length maintenance; the barbed ends were unaffected. When the thin filaments were depolymerized with latrunculin B, myocytes with decreased nebulin levels reassembled them to unrestricted lengths, demonstrating its importance in length specification. Finally, knockdown of nebulin in skeletal myotubes revealed its involvement in myofibrillogenesis. These data are consistent with nebulin functioning as a thin filament ruler and provide insight into mechanisms dictating macromolecular assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.