ArticleNeuron2005
The pore helix dipole has a minor role in inward rectifier channel function.
Article in Neuron, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 54 citations in OpenAlex.
- A selectivity filter mutation provides insights into gating regulation of a KCommunications biology · 2022Article
- A small viral potassium ion channel with an inherent inward rectification.Channels (Austin, Tex.) · 2019Article
- Mutation of a single residue promotes gating of vertebrate and invertebrate two-pore domain potassium channels.Nature communications · 2019Article
- Intrinsic Mechanisms of Frequency Selectivity in the Proximal Dendrites of CA1 Pyramidal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Review
- Inward rectifier potassium current (I K1) and Kir2 composition of the zebrafish (Danio rerio) heart.Pflugers Archiv : European journal of physiology · 2015Article
- The isoforms generated by alternative translation initiation adopt similar conformation in the selectivity filter in TREK-2.Journal of physiology and biochemistry · 2015Article
- Tethered protein display identifies a novel Kir3.2 (GIRK2) regulator from protein scaffold libraries.ACS chemical neuroscience · 2014Article
- Q/R site interactions with the M3 helix in GluK2 kainate receptor channels revealed by thermodynamic mutant cycles.The Journal of general physiology · 2013Article
- A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels.ACS chemical biology · 2013Article
- Role of methyl-induced polarization in ion binding.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Using yeast to study potassium channel function and interactions with small molecules.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Identification of functionally critical residues in the channel domain of inositol trisphosphate receptors.The Journal of biological chemistry · 2012Article
- External Ba2+ block of the two-pore domain potassium channel TREK-1 defines conformational transition in its selectivity filter.The Journal of biological chemistry · 2011Article
- Multiple modalities converge on a common gate to control K2P channel function.The EMBO journal · 2011Article
- A specific two-pore domain potassium channel blocker defines the structure of the TASK-1 open pore.The Journal of biological chemistry · 2011Article
- Distance- and activity-dependent modulation of spike back-propagation in layer V pyramidal neurons of the medial entorhinal cortex.Journal of neurophysiology · 2011Article
- Article
- Multi-scale electrophysiology modeling: from atom to organ.The Journal of general physiology · 2010Article
- Local control of postinhibitory rebound spiking in CA1 pyramidal neuron dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Ion channels lower the energetic barrier for ion passage across cell membranes and enable the generation of bioelectricity. Electrostatic interactions between permeant ions and channel pore helix dipoles have been proposed as a general mechanism for facilitating ion passage. Here, using genetic selections to probe interactions of an exemplar potassium channel blocker, barium, with the inward rectifier Kir2.1, we identify mutants bearing positively charged residues in the potassium channel signature sequence at the pore helix C terminus. We show that these channels are functional, selective, resistant to barium block, and have minimally altered conductance properties. Both the experimental data and model calculations indicate that barium resistance originates from electrostatics. We demonstrate that potassium channel function is remarkably unperturbed when positive charges occur near the permeant ions at a location that should counteract pore helix electrostatic effects. Thus, contrary to accepted models, the pore helix dipole seems to be a minor factor in potassium channel permeation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.