ArticleThe Journal of biological chemistry2005
HuR stabilizes vacuolar H+-translocating ATPase mRNA during cellular energy depletion.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- RNA-binding proteins and their role in kidney disease.Nature reviews. Nephrology · 2022Review
- Mammary epithelium-specific inactivation of V-ATPase reduces stiffness of extracellular matrix and enhances metastasis of breast cancer.Molecular oncology · 2018Article
- Loss of G2 subunit of vacuolar-type proton transporting ATPase leads to G1 subunit upregulation in the brain.Scientific reports · 2015Article
- Vacuolar ATPase in phagosome-lysosome fusion.The Journal of biological chemistry · 2015Article
- Krüppel -like factor 8 is a stress-responsive transcription factor that regulates expression of HuR.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2014Article
- Adaptive and maladaptive expression of the mRNA regulatory protein HuR.World journal of biological chemistry · 2013Review
- HuR inhibits apoptosis by amplifying Akt signaling through a positive feedback loop.Journal of cellular physiology · 2013Article
- Bioinformatic identification of novel elements potentially involved in messenger RNA fate control during spermatogenesis.Biology of reproduction · 2012Article
- Role of AUF1 and HuR in the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in LLC-PK₁-F⁺ cells.American journal of physiology. Renal physiology · 2011Article
- Myosin X regulates sealing zone patterning in osteoclasts through linkage of podosomes and microtubules.The Journal of biological chemistry · 2010Article
- Transcriptional control of human antigen R by bone morphogenetic protein.The Journal of biological chemistry · 2010Article
- Expression of the RNA-stabilizing protein HuR in ischemia-reperfusion injury of rat kidney.American journal of physiology. Renal physiology · 2009Article
- Regulated proteolysis of nonmuscle myosin IIA stimulates osteoclast fusion.The Journal of biological chemistry · 2009Article
- Article
- Tropomyosin 4 regulates adhesion structures and resorptive capacity in osteoclasts.Experimental cell research · 2008Article
- Renal response to metabolic acidosis: role of mRNA stabilization.Kidney international · 2008Review
- Expression and distribution of HuR during ATP depletion and recovery in proximal tubule cells.American journal of physiology. Renal physiology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
V-ATPases are multisubunit membrane proteins that use ATP binding and hydrolysis to transport protons across membranes against a concentration gradient. Although some cell types express plasma membrane forms of these transporters, all eukaryotes require V-ATPases to maintain an acidic pH in membrane-bound compartments of endocytic and secretory networks to facilitate protein trafficking and processing. Mammalian cells that completely lack V-ATPases are not viable; yet, the abundance of V-ATPases can differ among cell types by an order of magnitude or more, requiring precise control of their expression. We previously showed that mRNA stability appears to play a major role in regulating overall abundance of V-ATPases. In this report, we demonstrate that the stability of V-ATPase mRNA is regulated through AU-rich elements in 3'-untranslated regions. Unlike some mRNAs that are short-lived due to the presence of these elements, V-ATPase mRNAs have half-lives of hours to days. However, during stress induced by ATP depletion, AU-rich elements are necessary to maintain stability of these transcripts and their presence in the cytoplasm. HuR, an RNA-binding protein that interacts with and stabilizes AU-rich mRNAs, shows increased binding to some V-ATPase mRNAs during ATP depletion. siRNA-mediated knockdown of HuR results in diminished V-ATPase expression. These results indicate that AU-rich elements and associated proteins can play a role in regulation of even very stable mRNAs by protecting against loss during cellular stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.