ReviewCurrent opinion in structural biology2005
Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization.
Review in Current opinion in structural biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 170 citations in OpenAlex.
- Technical Scale in Biomolecular Crystallization.Advances in biochemical engineering/biotechnology · 2026Review
- Microfluidic Synthesis of -NHNanomaterials (Basel, Switzerland) · 2022Article
- Microfluidics as a Novel Technique for Tuberculosis: From Diagnostics to Drug Discovery.Microorganisms · 2021Review
- Droplet-based microfluidics for artificial cell generation: a brief review.Interface focus · 2016Review
- Calcium carbonate polymorph control using droplet-based microfluidics.Biomicrofluidics · 2012Article
- The Protein Structure Initiative Structural Biology Knowledgebase Technology Portal: a structural biology web resource.Journal of structural and functional genomics · 2012Article
- Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Challenges and opportunities for new protein crystallization strategies in structure-based drug design.Expert opinion on drug discovery · 2010Review
- Unmet challenges of structural genomics.Current opinion in structural biology · 2010Review
- Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.Journal of applied crystallography · 2010Article
- Image-based feedback control for real-time sorting of microspheres in a microfluidic device.Lab on a chip · 2010Article
- A Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases.Microfluidics and nanofluidics · 2010Article
- Multiparameter screening on SlipChip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods.Journal of the American Chemical Society · 2010Article
- High-throughput crystallography for structural genomics.Current opinion in structural biology · 2009Review
- Effects of impurities on membrane-protein crystallization in different systems.Acta crystallographica. Section D, Biological crystallography · 2009Article
- Facilitating protein crystal cryoprotection in thick-walled plastic capillaries by high-pressure cryocooling.Journal of applied crystallography · 2009Article
- Polymer nanofibre junctions of attolitre volume serve as zeptomole-scale chemical reactors.Nature chemistry · 2009Article
- Simultaneous generation of multiple aqueous droplets in a microfluidic device.Analytica chimica acta · 2008Article
- The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).Acta crystallographica. Section D, Biological crystallography · 2008Article
- Cryocrystallography in capillaries: critical glycerol concentrations and cooling rates.Journal of applied crystallography · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Protein crystallization is important for determining protein structures by X-ray diffraction. Nanoliter-sized plugs--aqueous droplets surrounded by a fluorinated carrier fluid--have been applied to the screening of protein crystallization conditions. Preformed arrays of plugs in capillary cartridges enable sparse matrix screening. Crystals grown in plugs inside a microcapillary may be analyzed by in situ X-ray diffraction. Screening using plugs, which are easily formed in PDMS microfluidic channels, is simple and economical, and minimizes consumption of the protein. This approach also has the potential to improve our understanding of the fundamentals of protein crystallization, such as the effect of mixing on the nucleation of crystals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.