Evidence map›Paper›PMID 16151142›Full record

ArticleApplied and environmental microbiology2005

Detection of diverse new Francisella-like bacteria in environmental samples.

Susan M Barns, Christy C Grow, Richard T Okinaka, Paul Keim, Cheryl R Kuske

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 157 citations in OpenAlex.

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  15. Exploring the Diversity Within the GenusFrontiers in microbiology · 2020
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  19. Tularemia as a waterborne disease: a review.Emerging microbes & infections · 2019
    Review
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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Susan M BarnsLos Alamos National Laboratory, Bioscience Division, Mail Stop M888, Los Alamos, NM 87545, USA.
Christy C Grow
Richard T Okinaka
Paul Keim
Cheryl R Kuske
Los Alamos National Laboratory · USNorthern Arizona University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Following detection of putative Francisella species in aerosol samples from Houston, Texas, we surveyed soil and water samples from the area for the agent of tularemia, Francisella tularensis, and related species. The initial survey used 16S rRNA gene primers to detect Francisella species and related organisms by PCR amplification of DNA extracts from environmental samples. This analysis indicated that sequences related to Francisella were present in one water and seven soil samples. This is the first report of the detection of Francisella-related species in soil samples by DNA-based methods. Cloning and sequencing of PCR products indicated the presence of a wide variety of Francisella-related species. Sequences from two soil samples were 99.9% similar to previously reported sequences from F. tularensis isolates and may represent new subspecies. Additional analyses with primer sets developed for detection and differentiation of F. tularensis subspecies support the finding of very close relatives to known F. tularensis strains in some samples. While the pathogenicity of these organisms is unknown, they have the potential to be detected in F. tularensis-specific assays. Similarly, a potential new subspecies of Francisella philomiragia was identified. The majority of sequences obtained, while more similar to those of Francisella than to any other genus, were phylogenetically distinct from known species and formed several new clades potentially representing new species or genera. The results of this study revise our understanding of the diversity and distribution of Francisella and have implications for tularemia epidemiology and our ability to detect bioterrorist activities.

Indexed as

Soil MicrobiologyWater MicrobiologyBacterial ProteinsDNA, BacterialDNA PrimersFlavoproteinsFrancisellaFrancisella tularensisGenetic VariationMolecular Sequence DataPolymerase Chain ReactionRNA, Ribosomal, 16SSequence Analysis, DNATularemiaBacterial ProteinsDNA, BacterialDNA PrimersFlavoproteinsRNA, Ribosomal, 16SSdhA protein, Bacteria

Identifiers

PMID16151142
PMCPMC1214603
OpenAlexW2152121968

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.