ArticleBiophysical journal2005
Molecular identification and reconstitution of depolarization-induced exocytosis monitored by membrane capacitance.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Non-ionotropic voltage-gated calcium channel signaling.Channels (Austin, Tex.) · 2024Review
- All-Optical Detection of Neuronal Membrane Depolarization in Live Cells Using Colloidal Quantum Dots.Nano letters · 2019Article
- The L-type Voltage-Gated Calcium Channel co-localizes with Syntaxin 1A in nano-clusters at the plasma membrane.Scientific reports · 2017Article
- Is it zippered? Does it flare? That darn complexin clamping SNARE.Biophysical journal · 2013Article
- Intra-membrane signaling between the voltage-gated Ca2+-channel and cysteine residues of syntaxin 1A coordinates synchronous release.Scientific reports · 2013Article
- The involvement of ser1898 of the human L-type calcium channel in evoked secretion.International journal of endocrinology · 2011Article
- Solar radiation induces non-nuclear perturbations and a false start to regulated exocytosis in Cryptosporidium parvum.PloS one · 2010Article
- Conformational changes induced in voltage-gated calcium channel Cav1.2 by BayK 8644 or FPL64176 modify the kinetics of secretion independently of Ca2+ influx.The Journal of biological chemistry · 2010Article
- Mechanisms of neuromodulation as dissected using Sr2+ at motor nerve endings.Journal of neurophysiology · 2008Article
- Depolarization-evoked secretion requires two vicinal transmembrane cysteines of syntaxin 1A.PloS one · 2007Article
- Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association.Biochemistry · 2007Article
- Control of depolarization-evoked presynaptic neurotransmitter release by Cav2.1 calcium channel: old story, new insights.Channels (Austin, Tex.)Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated exocytosis of neurotransmitters at synapses is fast and tightly regulated. It is unclear which proteins constitute the "minimal molecular machinery" for this process. Here, we show that a novel technique of capacitance monitoring combined with heterologous protein expression can be used to reconstitute exocytosis that is fast (<0.5 s) and triggered directly by membrane depolarization in Xenopus oocytes. Testing synaptic proteins, voltage-gated Ca2+ channels, and using botulinum and tetanus neurotoxins established that the expression of a Ca2+ channel together with syntaxin 1A, SNAP-25, and synaptotagmin was sufficient and necessary for the reconstitution of depolarization-induced exocytosis. Similar to synaptic exocytosis, the reconstituted release was sensitive to neurotoxins, modulated by divalent cations (Ca2+, Ba2+, and Sr2+) or channel (Lc-, N-type), and depended nonlinearly on divalent cation concentration. Because of its improved speed, native trigger, and great experimental versatility, this reconstitution assay provides a novel, promising tool to study synaptic exocytosis.
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