ArticleBiophysical journal2005
T-jump infrared study of the folding mechanism of coiled-coil GCN4-p1.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Temperature-jump solution X-ray scattering reveals distinct motions in a dynamic enzyme.Nature chemistry · 2019Article
- Influence of Membrane-Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Atomistic simulations indicate the functional loop-to-coiled-coil transition in influenza hemagglutinin is not downhill.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Unified understanding of folding and binding mechanisms of globular and intrinsically disordered proteins.Biophysical reviews · 2018Review
- Insights into Coupled Folding and Binding Mechanisms from Kinetic Studies.The Journal of biological chemistry · 2016Review
- Tightening up the structure, lighting up the pathway: Application of molecular constraints and light to manipulate protein folding, self-assembly and function.Science China. Chemistry · 2014Article
- How quickly can a β-hairpin fold from its transition state?The journal of physical chemistry. B · 2014Article
- Slow folding-unfolding kinetics of an octameric β-peptide bundle.ACS chemical biology · 2014Article
- Ultrafast hydrogen exchange reveals specific structural events during the initial stages of folding of cytochrome c.Journal of the American Chemical Society · 2014Article
- Highly anisotropic stability and folding kinetics of a single coiled coil protein under mechanical tension.Journal of the American Chemical Society · 2011Article
- The effects of pK(a) tuning on the thermodynamics and kinetics of folding: design of a solvent-shielded carboxylate pair at the a-position of a coiled-coil.Biophysical journal · 2010Article
- What lessons can be learned from studying the folding of homologous proteins?Methods (San Diego, Calif.) · 2010Review
- Probing the folding intermediate of Rd-apocyt b562 by protein engineering and infrared T-jump.Protein science : a publication of the Protein Society · 2007Article
- Molecular basis of coiled-coil formation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Dynamics, energetics, and structure in protein folding.Biochemistry · 2006Review
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Authors and funding
4 authors.
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Abstract
Partially folded intermediates have been frequently observed in equilibrium and kinetic protein folding studies. However, folding intermediates that exist at the native side of the rate-limiting step are rather difficult to study because they often evade detection by conventional folding kinetic methods. Here, we demonstrated that a laser-induced temperature-jump method can potentially be used to identify the existence of such post-transition or hidden intermediates. Specifically, we studied two cross-linked variants of GCN4-p1 coiled-coil. The GCN4 leucine zipper has been studied extensively and most of these studies have regarded it as a two-state folder. Our static circular dichroism and infrared data also indicate that the thermal unfolding of these two monomeric coiled-coils can be adequately described by an apparent two-state model. However, their temperature-jump-induced relaxation kinetics exhibit non-monoexponential behavior, dependent upon sequence and temperature. Taken together, our results support a folding mechanism wherein at least one folding intermediate populates behind the main rate-limiting step.
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