ArticleThe Journal of physiology2005
A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.Biology open · 2014Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Multivesicular exocytosis in rat pancreatic beta cells.Diabetologia · 2012Article
- Regulation of exocytic mode in hippocampal neurons by intra-bouton calcium concentration.The Journal of physiology · 2010Article
- Vesicular release mode shapes the postsynaptic response at hippocampal synapses.The Journal of physiology · 2009Article
- Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.Nature immunology · 2009Article
- A fluorescence-based technique to construct size distributions from single-object measurements: application to the extrusion of lipid vesicles.Biophysical journal · 2008Article
- Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells.The Journal of physiology · 2007Article
- Mechanisms of action of glucagon-like peptide 1 in the pancreas.Pharmacology & therapeutics · 2007Review
- Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla.The EMBO journal · 2006Article
- Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets.The Journal of physiology · 2006Article
- Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
- Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
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Authors and funding
6 authors.
Funding
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Abstract
We have developed an imaging approach to estimate the diameter of exocytic vesicles that are smaller than the resolution of an optical microscope and present within intact tissue. This approach is based on two-photon excitation imaging of polar tracers in the extracellular medium, is designated TEPIQ (two-photon extracellular polar-tracer imaging-based quantification), and has three variants. TEPIQ analysis of DeltaV measures vesicle volume with a fluid-phase tracer, sulforhodamine B (SRB). TEPIQ analysis of DeltaS determines vesicle surface area with a polar membrane tracer, FM1-43. TEPIQ analysis of DeltaV/DeltaS estimates vesicle diameter from the SRB/FM1-43 fluorescence ratio. TEPIQ analysis is insensitive to microscope settings because the same setup is used for calibration and actual experiments. We tested the validity of TEPIQ with glucose-induced exocytosis from beta-cells within pancreatic islets. The three TEPIQ variants yielded estimates for the mean diameter of exocytic vesicles of between 340 and 390 nm, consistent with the size of insulin granules. TEPIQ analysis relies on the combination of two-photon excitation imaging, the narrow intercellular spaces of intact tissue, and the presence of diffusible polar tracers in the extracellular medium. It allows quantitative imaging of exocytosis within secretory organs, yielding estimates of vesicle diameter with nanometer resolution.
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