ArticleThe Journal of physiology2005
Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.Molecular neurobiology · 2025Article
- Exocytosis, Endocytosis, and Their Coupling in Excitable Cells.Frontiers in molecular neuroscience · 2017Review
- Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabelling in cultured cells.European journal of histochemistry : EJH · 2013Article
- Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The role of de novo catecholamine synthesis in mediating methylmercury-induced vesicular dopamine release from rat pheochromocytoma (PC12) cells.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Syntaxin-3 regulates newcomer insulin granule exocytosis and compound fusion in pancreatic beta cells.Diabetologia · 2013Article
- Regulation of fusion pore closure and compound exocytosis in neuroendocrine PC12 cells by SCAMP1.Traffic (Copenhagen, Denmark) · 2011Article
- Characterization of sequential exocytosis in a human neuroendocrine cell line using evanescent wave microscopy and "virtual trajectory" analysis.European biophysics journal : EBJ · 2007Article
- Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells.The Journal of physiology · 2007Article
- Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla.The EMBO journal · 2006Article
- Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets.The Journal of physiology · 2006Article
- A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets.The Journal of physiology · 2005Article
- Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated exocytosis of PC12 cells using two-photon excitation imaging and extracellular polar tracers (TEP imaging) at the basal region of PC12 cells adjacent to the glass cover slip. TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis revealed that most exocytosis was mediated by large dense-core vesicles (LVs) with a mean diameter of 220 nm, and that exocytosis of LVs occurred slowly with a mean latency of approximately 7 s even though exocytosis was induced with large increases in cytosolic Ca2+ concentration by uncaging of a caged-Ca2+ compound. We also found that 97% of exocytic LVs remained poised at the plasma membrane, 72% maintained their fusion pores in an open conformation for more than 30 s, and 76% triggered sequential compound exocytosis of vesicles that were located deeper in the cytosol. Sequential compound exocytosis by PC12 cells was confirmed by electron microscopic investigation with photoconversion of diaminobenzidine by FM1-43 (a polar membrane tracer). Our data suggest that pre-stimulus docking of LVs to the plasma membrane does not necessarily hasten the fusion reaction, while docking and resulting stability of exocytic LVs facilitates sequential compound exocytosis, and thereby allowing mobilization of deep vesicles.
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