ArticleThe Journal of physiology2005
Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.Molecular neurobiology · 2025Article
- Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.Lab on a chip · 2018Article
- How intravesicular composition affects exocytosis.Pflugers Archiv : European journal of physiology · 2018Review
- An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.The EMBO journal · 2017Article
- Visualizing endocytotic pathways at transmission electron microscopy via diaminobenzidine photo-oxidation by a fluorescent cell-membrane dye.European journal of histochemistry : EJH · 2014Article
- Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabelling in cultured cells.European journal of histochemistry : EJH · 2013Article
- Exocytosis from pancreatic β-cells: mathematical modelling of the exit of low-molecular-weight granule content.Interface focus · 2011Article
- Unperturbed islet α-cell function examined in mouse pancreas tissue slices.The Journal of physiology · 2011Article
- Intravesicular factors controlling exocytosis in chromaffin cells.Cellular and molecular neurobiology · 2010Article
- Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.The Journal of biological chemistry · 2010Article
- Vesicle diffusion close to a membrane: intermembrane interactions measured with fluorescence correlation spectroscopy.Biophysical journal · 2008Article
- Dynamin I plays dual roles in the activity-dependent shift in exocytic mode in mouse adrenal chromaffin cells.Archives of biochemistry and biophysics · 2008Article
- Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells.The Journal of physiology · 2007Article
- Neuronal somatic ATP release triggers neuron-satellite glial cell communication in dorsal root ganglia.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Kiss-and-coat and compartment mixing: coupling exocytosis to signal generation and local actin assembly.Molecular biology of the cell · 2006Review
- Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla.The EMBO journal · 2006Article
- Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets.The Journal of physiology · 2006Article
- A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets.The Journal of physiology · 2005Article
- Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated exocytosis of PC12 cells using two-photon excitation imaging and extracellular polar tracers (TEP imaging) in the lateral membranes not facing the glass-cover slip. Upon photolysis of a caged Ca2+ compound, TEP imaging with FM1-43 (a polar membrane tracer) detected massive exocytosis of vesicles with a time constant of about 1 s. TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis revealed that the diameter of vesicles was small (55 nm). Extensive exocytosis of small vesicles (SVs) was shown to be mediated by the transient opening of a fusion pore with a diameter less than about 1.6 nm, and to be followed by direct ('kiss-and-run') endocytosis and translocation of the endocytic vesicles (EVs) deep into the cytoplasm. These processes were unaffected by GTP-gamma-S. In contrast, constitutive endocytic vesicles exhibited a diameter of 90 nm, took up molecules with a diameter of > 12 nm, and their formation was blocked by GTP-gamma-S. Electron-microscopic investigation with photoconversion of diaminobenzidine using FM1-43 confirmed an abundance of EVs with a diameter of 54 nm in stimulated cells. They rapidly translocated into the cytosol, and fused with endosomal organelles. The number of SV exocytosis events vastly exceeded the number of SVs morphologically docked at the plasma membrane. Simultaneous capacitance and FM1-43 measurements indicated that TEP imaging detected most SV exocytosis, and the fusion pore was closed within 2 s. Thus, we have, for the first time, directly visualized massive exocytosis of small vesicles in a non-synaptic preparation, and have revealed their fusion-pore mediated exocytosis and endocytosis.
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