ArticleProceedings of the National Academy of Sciences of the United States of America2005
Complex cardiac Nkx2-5 gene expression activated by noggin-sensitive enhancers followed by chamber-specific modules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Introduction to bioimaging-based spatial multi-omic novel methods.Quantitative biology (Beijing, China) · 2023Review
- Towards Improved HumanBiomedicines · 2023Review
- Novel enhancers conferring compensatory transcriptional regulation of Nkx2-5 in heart development.iScience · 2023Article
- (Tn5-)FISH-based imaging in the era of 3D/spatial genomics.Biophysics reports · 2023Article
- Spatiotemporal expression of long noncoding RNARNA biology · 2021Article
- Heart Enhancers: Development and Disease Control at a Distance.Frontiers in genetics · 2021Review
- Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.Stem cells (Dayton, Ohio) · 2014Article
- Coordinate Nodal and BMP inhibition directs Baf60c-dependent cardiomyocyte commitment.Genes & development · 2013Article
- Identifying DistalRSC advances · 2013Article
- GATA6 reporter gene reveals myocardial phenotypic heterogeneity that is related to variations in gap junction coupling.American journal of physiology. Heart and circulatory physiology · 2011Article
- Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons.BMC genomics · 2011Article
- T-box 2, a mediator of Bmp-Smad signaling, induced hyaluronan synthase 2 and Tgfbeta2 expression and endocardial cushion formation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Six2 activity is required for the formation of the mammalian pyloric sphincter.Developmental biology · 2009Article
- Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish.Nucleic acids research · 2008Article
- Genomic identification of regulatory elements by evolutionary sequence comparison and functional analysis.Advances in genetics · 2008Review
- Enhancer identification through comparative genomics.Seminars in cell & developmental biology · 2007Review
- The Kruppel-like transcription factor KLF13 is a novel regulator of heart development.The EMBO journal · 2006Article
- Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors.Nucleic acids research · 2005Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We previously reported that an Nkx2-5-GFP bacterial artificial chromosome in transgenic mice recapitulated the endogenous gene activity in the heart. Here, we identified three additional previously uncharacterized distal enhancer modules of Nkx2-5: UH6, which directed transgene expression in the right ventricle, interventricular septum, and atrial ventricular canal; UH5, which directed expression in both atria; and UH4, which directed transgene expression in tongue muscle. Nkx2-5 enhancers drive cardiogenic gene activity from the earliest progenitors to the late-stage embryonic heart, reside within its 27 kb of 5' flanking sequences, organized in a tandem array. Nkx2-5 enhancers involved with stomach-, tongue-, and chamber-restricted expression displayed lacZ transgene activity and chromatin histone acetylation patterns consistent with tissue-specific expression. An examination of Nkx2-5 gene activity in murine embryonic stem cells converted to beating embryoid bodies showed that only the proximal active region 2 and GATA-Smad enhancers were chromatin-remodeled. Chromatin remodeling of active region 2 and GATA-Smad enhancers were blunted by noggin coexpression, which indicated dependence on bone morphogenetic protein signaling for their chromatin activation during activation of Nkx2-5 expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.