ArticleThe Biochemical journal2005
Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- A Novel Calcium-Activated Potassium Channel Controls Membrane Potential and Intracellular pH inFrontiers in cellular and infection microbiology · 2019Article
- P-type transport ATPases in Leishmania and Trypanosoma.Parasite (Paris, France) · 2019Review
- Article
- Proteomic analysis of the acidocalcisome, an organelle conserved from bacteria to human cells.PLoS pathogens · 2014Article
- Rab11 regulates trafficking of trans-sialidase to the plasma membrane through the contractile vacuole complex of Trypanosoma cruzi.PLoS pathogens · 2014Article
- mAb CZP-315.D9: an antirecombinant cruzipain monoclonal antibody that specifically labels the reservosomes of Trypanosoma cruzi epimastigotes.BioMed research international · 2014Article
- Article
- Acylation-dependent export of Trypanosoma cruzi phosphoinositide-specific phospholipase C to the outer surface of amastigotes.The Journal of biological chemistry · 2010Article
- Two approaches to discovering and developing new drugs for Chagas disease.Memorias do Instituto Oswaldo Cruz · 2009Article
- Subcellular proteomics of Trypanosoma cruzi reservosomes.Proteomics · 2009Article
- All Trypanosoma cruzi developmental forms present lysosome-related organelles.Histochemistry and cell biology · 2008Article
- A Trypanosoma cruzi phosphatidylinositol 3-kinase (TcVps34) is involved in osmoregulation and receptor-mediated endocytosis.The Journal of biological chemistry · 2008Article
- Proteomics in Trypanosoma cruzi--localization of novel proteins to various organelles.Proteomics · 2008Article
- Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi.Molecular and biochemical parasitology · 2008Article
- Reservosomes: multipurpose organelles?Parasitology research · 2006Review
- Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies in Trypanosoma cruzi, the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes (TcHA1 and TcHA2) that encode P (phospho-intermediate form)-type H+-ATPases with homology to fungal and plant proton-pumping ATPases. In the present study, we demonstrate that these pumps are present in the plasma membrane and intracellular compartments of three different stages of T. cruzi. The main intracellular compartment containing these ATPases in epimastigotes was identified as the reservosome. This identification was achieved by immunofluorescence assays and immunoelectron microscopy showing their co-localization with cruzipain, and by subcellular fractionation and detection of their activity. ATP-dependent proton transport by isolated reservosomes was sensitive to vanadate and insensitive to bafilomycin A1, which is in agreement with the localization of P-type H+-ATPases in these organelles. Analysis by confocal immunofluorescence microscopy revealed that epitope-tagged TcHA1-Ty1 and TcHA2-Ty1 gene products are localized in the reservosomes, whereas the TcHA1-Ty1 gene product is additionally present in the plasma membrane. Immunogold electron microscopy showed the presence of the H+-ATPases in other compartments of the endocytic pathway such as the cytostome and endosomal vesicles, suggesting that in contrast with most cells investigated until now, the endocytic pathway of T. cruzi is acidified by a P-type H+-ATPase.
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