ArticleMagnetic resonance in medicine2005
Labeling fibroblasts with biotin-BSA-GdDTPA-FAM for tracking of tumor-associated stroma by fluorescence and MR imaging.
Article in Magnetic resonance in medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
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- Characterization of the Tumor Microenvironment and Tumor-Stroma Interaction by Non-invasive Preclinical Imaging.Frontiers in oncology · 2017Review
- Improving surface and defect center chemistry of fluorescent nanodiamonds for imaging purposes--a review.Analytical and bioanalytical chemistry · 2015Review
- Imaging aspects of the tumor stroma with therapeutic implications.Pharmacology & therapeutics · 2014Review
- Functional phenotyping of the maternal albumin turnover in the mouse placenta by dynamic contrast-enhanced MRI.Molecular imaging and biology · 2011Article
- Visualizing vascular permeability and lymphatic drainage using labeled serum albumin.Angiogenesis · 2010Review
- Distribution of CPP-Protein Complexes in Freshly Resected Human Tissue Material.Pharmaceuticals (Basel, Switzerland) · 2010Article
- Magnetic resonance imaging of cells in experimental disease models.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Harnessing competing endocytic pathways for overcoming the tumor-blood barrier: magnetic resonance imaging and near-infrared imaging of bifunctional contrast media.Cancer research · 2009Article
- Discrepancies between the fate of myoblast xenograft in mouse leg muscle and NMR label persistency after loading with Gd-DTPA or SPIOs.Gene therapy · 2009Article
- Mesenchymal stem cell-derived microvesicles protect against acute tubular injury.Journal of the American Society of Nephrology : JASN · 2009Article
- In vivo tracking of cellular therapeutics using magnetic resonance imaging.Expert opinion on biological therapy · 2009Review
- MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles.Magnetic resonance in medicine · 2008Article
- In vivo imaging of the systemic recruitment of fibroblasts to the angiogenic rim of ovarian carcinoma tumors.Cancer research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Fibroblasts at the tumor-host interface can differentiate into myofibroblasts and pericytes, and contribute to the guidance and stabilization of endothelial sprouts. After intravenous administration of biotin-BSA-GdDTPA-FAM in mice with subcutaneous MLS human ovarian carcinoma tumors, the distribution of the macromolecular MRI/optical contrast material was confined to blood vessels in normal tissues, while it co-registered with alphaSMA-positive stroma tracks within the tumor. These alphaSMA-positive tumor-associated myofibroblasts and pericytes showed uptake of the contrast material into intracellular granules. We evaluated the use of this contrast material for in vitro labeling of tumor fibroblasts as an approach for tracking their involvement in angiogenesis. Fluorescence microscopy demonstrated internalization of the contrast material, and MRI revealed a significant increase in the R(1) relaxation rate of labeled fibroblasts. R(1) not only remained elevated for 2 weeks in culture, it also increased with cell proliferation, indicating prolonged retention of the contrast material and subsequent intracellular processing and redistribution of the material, and thereby enhancing MR contrast. Moreover, cells that were labeled ex vivo with MR contrast material and co-inoculated with tumor cells in mice were detected in vivo by MRI. Uptake of the contrast material was suppressed by nystatin, suggesting internalization by caveolae-mediated endocytosis. This study shows that labeling of fibroblasts with biotin-BSA-GdDTPA-FAM is feasible and would allow noninvasive in vivo tracking of fibroblasts during tumor angiogenesis and vessel maturation.
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