ArticleClinical and diagnostic laboratory immunology2005
Avidity determinations for Haemophilus influenzae Type b anti-polyribosylribitol phosphate antibodies.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Avidity of anti-circumsporozoite antibodies following vaccination with RTS,S/AS01E in young children.PloS one · 2014Trial
- Immunologic response to Haemophilus influenzae type b (Hib) conjugate vaccine and risk factors for carriage among Hib carriers and noncarriers in Southwestern Alaska.Clinical and vaccine immunology : CVI · 2006Trial
- Comparing Traditional and Toxin-Oriented Approaches towards Antivenom Production againstToxins · 2023Article
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- Characterising antibody avidity in individuals of varied Mycobacterium tuberculosis infection status using surface plasmon resonance.PloS one · 2018Article
- Semisynthetic glycoconjugate vaccine candidate againstProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique.PLoS neglected tropical diseases · 2016Article
- Effects of Serial Skin Testing with Purified Protein Derivative on the Level and Quality of Antibodies to Complex and Defined Antigens in Mycobacterium bovis-Infected Cattle.Clinical and vaccine immunology : CVI · 2015Article
- Naturally acquired antibodies against Haemophilus influenzae type a in Aboriginal adults, Canada.Emerging infectious diseases · 2015Article
- Identification of antigen-specific B cell receptor sequences using public repertoire analysis.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Development and application of a GuHCl-modified ELISA to measure the avidity of anti-HPV L1 VLP antibodies in vaccinated individuals.Molecular and cellular probes · 2012Article
- Immunoglobulin G avidities in infants in Mexico after primary immunization with three doses of polyribosylribitol phosphate-tetanus toxoid Haemophilus influenzae type b vaccine.Clinical and vaccine immunology : CVI · 2008Article
- Avidity of the immunoglobulin G response to a Neisseria meningitidis group C polysaccharide conjugate vaccine as measured by inhibition and chaotropic enzyme-linked immunosorbent assays.Clinical and vaccine immunology : CVI · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of antibody avidity measurements can be difficult in human serum depending on the population evaluated. We evaluated three approaches for the determination of antibody avidity for immunoglobulin G (IgG). These approaches were (i) elution of bound antibody with increasing concentrations of a chaotropic agent using a single serum dilution, (ii) binding interference of multiple serum dilutions by a single concentration of a chaotrope, and (iii) elution of multiple serum dilutions by a single concentration of a chaotrope. Parameters that affect the determination of avidity measurements and their limitations were evaluated with pre- and post-Haemophilus influenzae type b conjugate vaccination sera (n=89). We determined that elution of low-avidity antibodies present in multiple dilutions of the serum sample by a single concentration of a chaotrope (0.15 M sodium thiocyanate [NaSCN]) was optimal for the determination of avidity measurements throughout a wide range of IgG concentrations (0.94 to 304.6 microg/ml). The percent reduction in concentration as determined by the elution assay with 0.15 M NaSCN correlated highly (r=0.84) with weighted averages obtained by an elution assay with multiple solutions of NaSCN. The correlation (r=0.57) between elution and binding interference, when a single concentration of a chaotrope was used, was lower than the correlation between the two elution methods (r=0.84). We found that the serum dilution, the heterogeneity of the antibody population, and the concentration of the chaotrope were the primary variables affecting avidity determinations. In this study, we present multiple analysis methods depending on the methodology used. We also present the factors that affect the analysis of avidity determinations given the polyclonal nature of human sera. This experimental approach should benefit the evaluation of similar antibodies induced by other bacterial polysaccharide vaccines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.