ArticleMolecular biology of the cell2005
Drosophila Nod protein binds preferentially to the plus ends of microtubules and promotes microtubule polymerization in vitro.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Identification of key residues that regulate the interaction of kinesins with microtubule ends.Cytoskeleton (Hoboken, N.J.) · 2019Article
- NOD is a plus end-directed motor that binds EB1 via a new microtubule tip localization sequence.The Journal of cell biology · 2018Article
- Kinesin 6 Regulation inG3 (Bethesda, Md.) · 2018Article
- The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition.Open biology · 2016Article
- WDR73 Mutations Cause Infantile Neurodegeneration and Variable Glomerular Kidney Disease.Human mutation · 2015Article
- Mitosis, microtubule dynamics and the evolution of kinesins.Experimental cell research · 2015Review
- Meiosis: an overview of key differences from mitosis.Cold Spring Harbor perspectives in biology · 2015Review
- The Ran Pathway in Drosophila melanogaster Mitosis.Frontiers in cell and developmental biology · 2015Review
- Nondisjunctional segregations in Drosophila female meiosis I are preceded by homolog malorientation at metaphase arrest.Genetics · 2013Article
- Elevated polar ejection forces stabilize kinetochore-microtubule attachments.The Journal of cell biology · 2013Article
- Coupling of kinesin ATP turnover to translocation and microtubule regulation: one engine, many machines.Journal of muscle research and cell motility · 2012Review
- Move in for the kill: motile microtubule regulators.Trends in cell biology · 2012Review
- A germline clone screen on the X chromosome reveals novel meiotic mutants in Drosophila melanogaster.G3 (Bethesda, Md.) · 2012Article
- Unconventional functions of microtubule motors.Archives of biochemistry and biophysics · 2012Review
- Dusky-like functions as a Rab11 effector for the deposition of cuticle during Drosophila bristle development.Development (Cambridge, England) · 2012Article
- A metal switch for controlling the activity of molecular motor proteins.Nature structural & molecular biology · 2011Article
- Mitotic kinesin CENP-E promotes microtubule plus-end elongation.Current biology : CB · 2010Article
- How kinesin motor proteins drive mitotic spindle function: Lessons from molecular assays.Seminars in cell & developmental biology · 2010Review
- The impact on microtubule network of a bracovirus IkappaB-like protein.Cellular and molecular life sciences : CMLS · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Nod, a nonmotile kinesin-like protein, plays a critical role in segregating achiasmate chromosomes during female meiosis. In addition to localizing to oocyte chromosomes, we show that functional full-length Nod-GFP (Nod(FL)-GFP) localizes to the posterior pole of the oocyte at stages 9-10A, as does kinesin heavy chain (KHC), a plus end-directed motor. This posterior localization is abolished in grk mutants that no longer maintain the microtubule (MT) gradient in the oocyte. To test the hypothesis that Nod binds to the plus ends of MTs, we expressed and purified both full-length Nod (Nod(FL)) and a truncated form of Nod containing only the motor-like domain (Nod318) from Escherichia coli and assessed their interactions with MTs in vitro. Both Nod(FL) and Nod318 demonstrate preferential binding to the ends of the MTs, displaying a strong preference for binding to the plus ends. When Nod318-GFP:MT collision complexes were trapped by glutaraldehyde fixation, the preference for binding to plus ends versus minus ends was 17:1. Nod(FL) and Nod318 also promote MT polymerization in vitro in a time-dependent manner. The observation that Nod is preferentially localized to the plus ends of MTs and stimulates MT polymerization suggests a mechanism for its function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.