ArticleMolecular and cellular probes2005
Clean Western blot signals from immunoprecipitated samples.
Article in Molecular and cellular probes, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
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- Quantitation of norovirus-specific IgG before and after infection in immunocompromised patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Mitragynine, an euphoric compound inhibits hERG1a/1b channel current and upregulates the complexation of hERG1a-Hsp90 in HEK293-hERG1a/1b cells.Scientific reports · 2019Article
- An overview of technical considerations for Western blotting applications to physiological research.Scandinavian journal of medicine & science in sports · 2017Review
- Efficacy and Mechanism of Action of Low Dose Emetine against Human Cytomegalovirus.PLoS pathogens · 2016Article
- β-arrestin-1 drives endothelin-1-mediated podocyte activation and sustains renal injury.Journal of the American Society of Nephrology : JASN · 2014Article
- Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae.Microbial cell factories · 2014Article
- β Integrins mediate FAK Y397 autophosphorylation of resistance arteries during eutrophic inward remodeling in hypertension.Journal of vascular research · 2014Article
- Thylakoid FtsH protease contributes to photosystem II and cytochrome b6f remodeling in Chlamydomonas reinhardtii under stress conditions.The Plant cell · 2014Article
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- LPS-induced production of TNF-α and IL-6 in mast cells is dependent on p38 but independent of TTP.Cellular signalling · 2013Article
- Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis.PloS one · 2013Article
- Membrane localization of membrane type 1 matrix metalloproteinase by CD44 regulates the activation of pro-matrix metalloproteinase 9 in osteoclasts.BioMed research international · 2013Article
- MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors.The Journal of cell biology · 2012Article
- β-arrestin1 mediates metastatic growth of breast cancer cells by facilitating HIF-1-dependent VEGF expression.Oncogene · 2012Article
- Analysis of the bioactivity of magnetically immunoisolated peroxisomes.Analytical and bioanalytical chemistry · 2012Article
- Recruitment of SWI/SNF complex is required for transcriptional activation of the SLC11A1 gene during macrophage differentiation of HL-60 cells.The Journal of biological chemistry · 2011Article
- Regulation of trafficking of activated TrkA is critical for NGF-mediated functions.Traffic (Copenhagen, Denmark) · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We present a strategy that overcomes the high background arising during Western blotting (WB) detection of proteins obtained through immunoprecipitation (IP). Traditional HRP-conjugated secondary antibodies, which detect the denatured heavy and light antibody chains, produce high background that often mask the signals of interest on WBs. Here, we show that HRP-conjugated Protein A and Protein G, which detect almost exclusively intact antibody molecules, can be effectively used to obtain clean and specific WB signals of target proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.