ArticleTransgenic research2005
Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack.
Article in Transgenic research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 66 citations in OpenAlex.
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis.Transgenic research · 2023Article
- DestructiveFrontiers in microbiology · 2023Review
- Advances and prospects of orchid research and industrialization.Horticulture research · 2022Article
- Ovule Development andInternational journal of molecular sciences · 2020Article
- Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene.Botanical studies · 2020Article
- Genome-wide analysis of small RNAs from Odontoglossum ringspot virus and Cymbidium mosaic virus synergistically infecting Phalaenopsis.Molecular plant pathology · 2020Article
- Dual resistance of transgenic plants against Cymbidium mosaic virus and Odontoglossum ringspot virus.Scientific reports · 2019Article
- Constitutive expression of a plant ferredoxin-like protein (pflp) enhances capacity of photosynthetic carbon assimilation in rice (Oryza sativa).Transgenic research · 2017Article
- Efficient and heritable transformation of Phalaenopsis orchids.Botanical studies · 2016Article
- Functional stacking of three resistance genes against Phytophthora infestans in potato.Transgenic research · 2012Article
- Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids.BMC genomics · 2011Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Agrobacterium-mediated transformation of protocorm-like bodies in Cymbidium.Plant cell reports · 2007Article
- Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation.Planta · 2007Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cymbidium Mosaic Virus (CymMV) and Erwinia carotovora have been reported to cause severe damage to orchid plants. To enhance the resistance of orchids to both viral and bacterial phytopathogens, gene stacking was applied on Phalaenopsis orchid by double transformation. PLBs originally transformed with CymMV coat protein cDNA (CP) were then re-transformed with sweet pepper ferredoxin-like protein cDNA (Pflp) by Agrobacterium tumefaciens, to enable expression of dual (viral and bacterial) disease resistant traits. A non-antibiotic selection procedure in the second transformation minimized the potential rate of 'stacking' antibiotic genes in the orchid gene pool. Transgene integration in transgenic Phalaenopsis lines was confirmed by Southern blot analysis for both CP and pflp genes. Expression of transgenes was detected by northern blot analysis, and disease resistant assays revealed that transgenic lines exhibited enhanced resistance to CymMV and E. carotovora. This is the first report describing a transgenic Phalaenopsis orchid with dual resistance to phytopathogens.
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