ArticleBiological procedures online2005
Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimulons and regulons in bacteria with sequenced or partially sequenced genomes.
Article in Biological procedures online, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Protein Architecture and Composition in Mycobacterium tuberculosis.Advances in experimental medicine and biology · 2026Review
- Exosome-based therapies for inflammatory disorders: a review of recent advances.Stem cell research & therapy · 2024Review
- A systematic evaluation of sample preparation and 2-D gel electrophoresis protocol for mosquito proteomic profiling.MethodsX · 2024Article
- Impact of c-di-GMP on the Extracellular Proteome ofBiology · 2022Article
- Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Potential Use of Exosomes as Diagnostic Biomarkers and in Targeted Drug Delivery: Progress in Clinical and Preclinical Applications.ACS biomaterials science & engineering · 2021Review
- The CRISPR-Cas System Is Involved in OmpR Genetic Regulation for Outer Membrane Protein Synthesis inFrontiers in microbiology · 2021Article
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- Challenges and opportunities in exosome research-Perspectives from biology, engineering, and cancer therapy.APL bioengineering · 2019Review
- Infection with Plasmodium berghei ookinetes alters protein expression in the brain of Anopheles albimanus mosquitoes.Parasites & vectors · 2016Article
- Article
- Data for a comparative proteomic analysis of chloroplast biogenesis (clb) mutants.Data in brief · 2014Article
- A PPARγ, NF-κB and AMPK-dependent mechanism may be involved in the beneficial effects of curcumin in the diabetic db/db mice liver.Molecules (Basel, Switzerland) · 2014Article
- Mice deficient in dual oxidase maturation factors are severely hypothyroid.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Proteomic analysis of Bacillus thuringiensis strain 4.0718 at different growth phases.TheScientificWorldJournal · 2012Article
- Systems biology of bacterial nitrogen fixation: high-throughput technology and its integrative description with constraint-based modeling.BMC systems biology · 2011Article
- argC Orthologs from Rhizobiales show diverse profiles of transcriptional efficiency and functionality in Sinorhizobium meliloti.Journal of bacteriology · 2011Article
- Characterization of the NifA-RpoN regulon in Rhizobium etli in free life and in symbiosis with Phaseolus vulgaris.Applied and environmental microbiology · 2010Article
- The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to define the protein components of regulons and stimulons in bacteria, including those organisms where genome sequencing is still in progress. The basic 2-DE protocol allows high resolution and reproducibility and enables the direct comparison of hundreds or even thousands of proteins simultaneously. To identify proteins that comprise stimulons and regulons, peptide mass fingerprint (PMF) with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS) analysis is the first option and, if results from this tool are insufficient, complementary data obtained with electrospray ionization tandem-MS (ESI-MS/MS) may permit successful protein identification. ESI-MS/MS and MALDI-TOF-MS provide complementary data sets, and so a more comprehensive coverage of a proteome can be obtained using both techniques with the same sample, especially when few sequenced proteins of a particular organism exist or genome sequencing is still in progress.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.