ArticleJournal of clinical microbiology2005
Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target.Pathogens (Basel, Switzerland) · 2026Article
- Sample preparation and detection methods in point-of-care devices towards future at-home testing.Lab on a chip · 2024Review
- Is an oropharyngeal HPV infection more frequently detectable in women with a genital HPV infection?European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2024Article
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- Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.Analytical biochemistry · 2018Article
- Characterization ofNew microbes and new infections · 2017Article
- Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection.PloS one · 2016Article
- Comparison of an automated nucleic acid extraction system with the column-based procedure.European journal of microbiology & immunology · 2015Article
- The Rapid-Heat LAMPellet Method: A Potential Diagnostic Method for Human Urogenital Schistosomiasis.PLoS neglected tropical diseases · 2015Article
- Comparison of sputum and nasopharyngeal aspirate samples and of the PCR gene targets lytA and Spn9802 for quantitative PCR for rapid detection of pneumococcal pneumonia.Journal of clinical microbiology · 2014Article
- Article
- Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools.Journal of virological methods · 2013Article
- Respiratory virus multiplex RT-PCR assay sensitivities and influence factors in hospitalized children with lower respiratory tract infections.Virologica Sinica · 2013Article
- Quality assurance program for molecular medicine laboratories.Iranian journal of public health · 2013Article
- Long-term frozen storage of urine samples: a trouble to get PCR results in Schistosoma spp. DNA detection?PloS one · 2013Article
- Immune mediators of protective and pathogenic immune responses in patients with mild and fatal human monocytotropic ehrlichiosis.BMC immunology · 2012Article
- Simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Article
- Detection and identification of Ehrlichia species in blood by use of PCR and electrospray ionization mass spectrometry.Journal of clinical microbiology · 2010Article
- C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens.Journal of clinical microbiology · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative validation on three systems: the MagNA Pure compact system (Compact), the NucliSens miniMAG extraction instrument (miniMAG), and the BioRobot EZ1 system (EZ1). A total of 75 urine specimens submitted for polyomavirus BK virus detection were used. The human beta-actin gene was detected on 75 (100%), 75 (100%), and 72 (96%) nucleic acid extracts prepared by the miniMAG, EZ1, and Compact, respectively. The miniMAG produced the highest quantity of nucleic acids and the best precision among the three systems. The agreement rate was 100% for BKV detection on nucleic acid extracts prepared by the three extraction systems. When a full panel of specimens was run, the hands-on time and test turnaround time were 105.7 and 121.1 min for miniMAG, 6.1 and 22.6 min for EZ1, and 7.4 and 33.7 min for Compact, respectively. The EZ1 and Compact systems processed automatic nucleic acid extraction properly, providing a good solution to the need for sporadic but emergent specimen detection. The miniMAG yielded the highest quantity of nucleic acids, suggesting that this system would be the best for specimens containing a low number of microorganisms of interest.
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