ArticleJournal of clinical microbiology2005
Use of rapid dipstick and latex agglutination tests and enzyme-linked immunosorbent assay for serodiagnosis of amebic liver abscess, amebic Colitis, and Entamoeba histolytica Cyst Passage.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- The Abscess That Was Cancer: Pyogenic Liver Abscess as the Initial Manifestation of Hepatocellular Carcinoma in a Noncirrhotic Patient.Case reports in hepatology · 2026Article
- Serodiagnosis of amoebic abscess: a retrospective diagnostic accuracy study of kits marketed in Europe.Journal of clinical microbiology · 2025Article
- Article
- Diagnostic Methods of Common Intestinal Protozoa: Current and Future Immunological and Molecular Methods.Tropical medicine and infectious disease · 2022Review
- Accurate laboratory diagnosis of human intestinal and extra-intestinal amoebiasis.Gastroenterology and hepatology from bed to bench · 2022Review
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- Aptamer-based approaches for the detection of waterborne pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Update on laboratory diagnosis of amoebiasis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Indirect Haemagglutination Test in Comparison with ELISA for Detection of Antibodies against Invasive Amoebiasis.Journal of clinical and diagnostic research : JCDR · 2016Article
- Rapid Diagnosis of Intestinal Parasitic Protozoa, with a Focus on Entamoeba histolytica.Interdisciplinary perspectives on infectious diseases · 2009Article
- Laboratory diagnostic techniques for Entamoeba species.Clinical microbiology reviews · 2007Review
- Evaluation of Entamoeba histolytica antigen and antibody point-of-care tests for the rapid diagnosis of amebiasis.Journal of clinical microbiology · 2006Article
- Seroprevalence of Entamoeba histolytica from a tertiary care hospital, South India.Tropical parasitologyArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A homemade enzyme-linked immunosorbent assay (ELISA) and a dipstick assay (Dipstick) for the detection of anti-Entamoeba histolytica antibodies in serum were developed and evaluated together with a commercially available latex agglutination test (LAT; Laboratoires Fumouze) for their use in serodiagnosis of amebiasis. The sensitivity of these assays was evaluated with sera from 27 patients with radiologically proven, cellulose acetate precipitation (CAP) test-positive amebic liver abscess, 7 patients with parasitologically and PCR-proven amebic colitis, and 11 patients with parasitologically and PCR-proven E. histolytica cyst passage. The sensitivities of the ELISA, Dipstick, and LAT were all 93.3% (42/45). With a combination of Dipstick and LAT, all abscess and colitis patients had at least one positive result. The specificity was assessed with 238 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases, sera containing autoimmune antibodies, and sera from healthy blood donors. The specificities of the ELISA, Dipstick, and LAT were 97.1%, 98.1%, and 99.5%, respectively. Of 61 sera from patients with PCR-proven E. dispar infection, 60 (98.4%) were negative in both Dipstick and LAT and 59 (96.7%) were negative in ELISA. Our data suggest that all three assays are sensitive serological tests. The rapid LAT and Dipstick provide fast results and can easily be applied in routine laboratories in order to facilitate the diagnosis of amebiasis.
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