ArticleJournal of clinical microbiology2005
Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Can We Improve Antifungal Susceptibility Testing?Frontiers in cellular and infection microbiology · 2021Review
- Evaluation of antifungal activity of cinnamaldehyde against Cryptococcus neoformans var. grubii.Folia microbiologica · 2020Article
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
- Unveiling the Synergistic Interaction Between Liposomal Amphotericin B and Colistin.Frontiers in microbiology · 2016Article
- A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases.Frontiers in microbiology · 2016Article
- New Insights Regarding Yeast Survival following Exposure to Liposomal Amphotericin B.Antimicrobial agents and chemotherapy · 2015Article
- Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry.Memorias do Instituto Oswaldo Cruz · 2014Article
- The effect of cinnamaldehyde on the growth and the morphology of Candida albicans.Medical molecular morphology · 2013Article
- Detection of Legionella pneumophila on clinical samples and susceptibility assessment by flow cytometry.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Comparison of the Etest and a rapid flow cytometry-based method with the reference CLSI broth microdilution protocol M27-A3 for the echinocandin susceptibility testing of Candida spp.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Rapid susceptibility testing and microcolony analysis of Candida spp. cultured and imaged on porous aluminum oxide.PloS one · 2012Article
- Cytometric approach for detection of Encephalitozoon intestinalis, an emergent agent.Clinical and vaccine immunology : CVI · 2009Article
- Propofol lipidic infusion promotes resistance to antifungals by reducing drug input into the fungal cell.BMC microbiology · 2008Article
- Antifungal effects on metabolite profiles of medically important yeast species measured by nuclear magnetic resonance spectroscopy.Antimicrobial agents and chemotherapy · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cytometric approach to determine the susceptibilities of Candida spp. and Cryptococcus neoformans to voriconazole, itraconazole, and caspofungin is described. A total of 63 clinical isolates with different susceptibility patterns were exposed for 1, 2, 4, and 6 h to serial concentrations of each antifungal agent, followed by staining with two fluorescent probes: propidium iodide (PI) and FUN-1. FUN-1 was able to identify the susceptibility patterns of the assayed strains to the three agents after 1 h. PI penetrated a maximum of 50% of the cells treated with PI, at the highest concentration of caspofungin, 16 mug/ml, after 6 h of incubation (this percentage varied with the strain and was drug concentration and time of incubation dependent) and did not stain cells treated with high concentrations of either azole after 6 h. The use of FUN-1 appears to be an excellent fast and reliable alternative to the classical dilution method for determining the susceptibility of Candida spp. and C. neoformans to these three antifungal agents.
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