ArticleJournal of clinical microbiology2005
Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
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Who cites it
95 citing papers in PubMed.
- Burkholderia arboris bacteremia initially identified as Burkholderia cepacia complex: a genome-based case report.Archives of microbiology · 2026Article
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- Laboratory strategies for the identification of Burkholderia species: From classical phenotyping to advanced genomic and proteomic approaches.Antonie van Leeuwenhoek · 2026Review
- Introgression impacts the evolution of bacteria, but species borders are rarely fuzzy.Nature communications · 2025Article
- Complete closed genomes of fourMicrobiology resource announcements · 2025Article
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- Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogenMicrobiology (Reading, England) · 2023Article
- Specific biomarker mining and rapid detection ofFrontiers in microbiology · 2023Article
- Soleris® Automated System for the Rapid Detection of Burkholderia cepacia Complex in Cosmetic Products.Journal of AOAC International · 2022Article
- The extracellular innate-immune effector HMGB1 limits pathogenic bacterial biofilm proliferation.The Journal of clinical investigation · 2021Article
- Burkholderia species in human infections in Mexico: Identification of B. cepacia, B. contaminans, B. multivorans, B. vietnamiensis,B. pseudomallei and a new Burkholderia species.PLoS neglected tropical diseases · 2021Article
- Genomics reveals the novel species placement of industrial contaminant isolates incorrectly identified asMicrobial genomics · 2021Article
- Genomics of an endemic cystic fibrosis Burkholderia multivorans strain reveals low within-patient evolution but high between-patient diversity.PLoS pathogens · 2021Article
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- Genetic Diversity and Characterization of Symbiotic Bacteria Isolated from Endemic Phaseolus Cultivars Located in Contrasting Agroecosystems in Venezuela.Microbes and environments · 2021Article
- Comparison of Microbiological Characteristics and Genetic Diversity betweenMicroorganisms · 2020Article
- A Phylogeny-Informed Proteomics Approach for Species Identification within the Burkholderia cepacia Complex.Journal of clinical microbiology · 2020Article
35 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
A single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least nine closely related species. Seven conserved housekeeping genes were selected after a comparison of five Burkholderia species, and a collection of strains was subjected to nucleotide sequence analysis using a nested PCR amplification approach for each gene. MLST differentiated all nine current BCC species and identified 114 sequence types within a collection of 119 strains. No differentiation was found between strains recovered from environmental or clinical sources. The improved resolution in strain identification offered by MLST was able to identify previously characterized epidemic strain lineages and also demonstrated the presence of four novel potential species groups within the complex. There was also evidence for recombination having an important role in the recent evolution of individual BCC species. This highly transferable, validated, MLST scheme provides a new means to assist in species identification as well as unambiguous strain discrimination of the BCC by a single approach. It is also the first MLST scheme designed at the outset to incorporate multiple species and should facilitate global epidemiological investigations of the BCC.
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