ArticleStem cells (Dayton, Ohio)2005
Bypass of senescence, immortalization, and transformation of human hematopoietic progenitor cells.
Article in Stem cells (Dayton, Ohio), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Stem cell factor and erythropoietin-independent production of cultured reticulocytes.Haematologica · 2024Article
- Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application.Stem cell research & therapy · 2023Review
- Immortalized striatal precursor neurons from Huntington's disease patient-derived iPS cells as a platform for target identification and screening for experimental therapeutics.Human molecular genetics · 2021Article
- Direct reprogramming of human Sertoli cells into male germline stem cells with the self-renewal and differentiation potentials via overexpressing DAZL/DAZ2/BOULE genes.Stem cell reports · 2021Article
- A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration.Cell & bioscience · 2019Review
- Non-invasiveOncotarget · 2017Article
- Fluorescent Proteins for Flow Cytometry.Current protocols in cytometry · 2017Article
- Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.Oncotarget · 2017Article
- Evolution of malignant plasmacytoma cell lines from K14E7 Fancd2-/- mouse long-term bone marrow cultures.Oncotarget · 2016Article
- Cardiac Stem Cell Secretome Protects Cardiomyocytes from Hypoxic Injury Partly via Monocyte Chemotactic Protein-1-Dependent Mechanism.International journal of molecular sciences · 2016Article
- Establishment and Characterization of Human Germline Stem Cell Line with Unlimited Proliferation Potentials and no Tumor Formation.Scientific reports · 2015Article
- SIRT1 inhibition affects angiogenic properties of human MSCs.BioMed research international · 2014Article
- MePR: a novel human mesenchymal progenitor model with characteristics of pluripotency.Stem cells and development · 2013Article
- Establishment of an erythroid cell line from primary CD36+ erythroid progenitor cells.Experimental hematology · 2010Article
- TLX1 and NOTCH coregulate transcription in T cell acute lymphoblastic leukemia cells.Molecular cancer · 2010Article
- Hematopoietic immortalizing function of the NKL-subclass homeobox gene TLX1.Genes, chromosomes & cancer · 2010Article
- TLX1/HOX11-induced hematopoietic differentiation blockade.Oncogene · 2007Article
- Telomere length of cord blood-derived CD34(+) progenitors predicts erythroid proliferative potential.Leukemia · 2007Article
- Evidence for a stem cell hierarchy in the adult human breast.The Journal of cell biology · 2007Article
- Stable gammaretroviral vector expression during embryonic stem cell-derived in vitro hematopoietic development.Molecular therapy : the journal of the American Society of Gene Therapy · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We attempted to extend the lifespan of CD34+ stem/progenitor cells in human cord blood (CB) by transduction with lentiviral vectors carrying the human telomerase catalytic subunit (hTERT) and/or the human papillomavirus type 16 (HPV16) E6 and E7 oncogenes. We found that hTERT was incapable of prolonging the replicative capacity of CB cells maintained under serum-free conditions in the presence of stem cell factor, Flt3 ligand, thrombopoietin, and interleukin-3 beyond 4 months (n=3). However, transduced CB cells cultured in the same cytokine cocktail constitutively expressing HPV16 E6/E7 alone (n=2) or in concert with hTERT (n=9) continued to proliferate, giving rise to permanent (>2 years) cell lines with a CD45+ CD34- CD133+/- CD44+ CD235a+ CD71+ CD203+ CD33+ CD13+ myeloerythroid/mast cell progenitor phenotype. Notably, CB cell cultures expressing only HPV16 E6/E7 went through a crisis period, and the resulting oligoclonal cell lines were highly aneuploid. By comparison, the CB cell lines obtained by coexpression of HPV16 E6/E7 plus hTERT exhibited near-diploid karyotypes with minimal chromosomal aberrations, concomitant with stabilization of telomere length, yet were clonally derived. The immortalized E6/E7 plus hTERT-expressing CB cells were not tumorigenic when injected intravenously or subcutaneously into sublethally irradiated immunodeficient nonobese diabetic/severe combined immunodeficient mice but could be converted to a malignant state by ectopic expression of a v-H-ras or BCR-ABL oncogene. These findings provide new insights into the mechanisms governing the senescence checkpoint of primitive human hematopoietic precursors and establish a paradigm for studies of the multistep process of human leukemogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.