ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Development of a molecular marker specific to a novel CMS line in radish (Raphanus sativus L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 40 citations in OpenAlex.
- Genetic Mechanisms for Hybrid Breeding in Vegetable Crops.Plants (Basel, Switzerland) · 2023Review
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Organelle Comparative Genome Analysis Reveals Novel Alloplasmic Male Sterility withInternational journal of molecular sciences · 2021Article
- An insertion mutation located on putative enhancer regions of theMolecular breeding : new strategies in plant improvement · 2021Article
- Appearance of male sterile and black radishes in the progeny of cross betweenBreeding science · 2020Article
- Mitochondrial Genome Sequencing RevealsGenes · 2020Article
- Article
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- Comparative Transcriptome Profile of the Cytoplasmic Male Sterile and Fertile Floral Buds of Radish (Raphanus sativus L.).International journal of molecular sciences · 2016Article
- Cytoplasmic male sterility in Brassicaceae crops.Breeding science · 2014Review
- Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance.Journal of experimental botany · 2013Article
- Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis.Breeding science · 2011Article
- Identification of mitochondrial genome rearrangements unique to novel cytoplasmic male sterility in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Discovery of a novel cytoplasmic male-sterility and its restorer lines in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- A new fertility restorer locus linked closely to the Rfo locus for cytoplasmic male sterility in radish.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we have investigated the cytoplasmic male sterility (CMS) of a novel male sterile radish line, designated NWB CMS. The NWB CMS was crossed with 16 fertile breeding lines, and all the progenies were completely male sterile. The degree of male sterility exhibited by NWB CMS is more than Ogura CMS from the Cruciferae family. The NWB CMS was found to induce 100% male sterility when crossed with all the tested breeding lines, whereas the Ogura CMS did not induce male sterility with any of the breeding lines. PCR analysis revealed that the molecular factor that influenced Ogura CMS, the orf138 gene, was absent in the NWB CMS line, and that the orf138 gene was not also expressed in this CMS line. In order to identify the cytoplasmic factors that confer male sterility in the NWB CMS line, we carried out RFLP analyses with 32 mitochondrial genes, all of which were used as probes. Fourteen genes exhibited polymorphisms between the NWB CMS line and other radish cultivars. Based on these RFLP data, intergenic primers were developed in order to amplify the intergenic regions between the polymorphic genes. Among these, a primer pair at the 3' region of the atp6 gene (5'-cgcttggactatgctatgtatga-3') and the 5' region of the nad3 gene (5'-tcatagagaaatccaatcgtcaa-3') produced a 2 kbp DNA fragment as a result of PCR. This DNA fragment was found to be specific to NWB CMS and was not present in other CMS types. It appears that this fragment could be used as a DNA marker to select NWB CMS line in a radish-breeding program.
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