ArticleJournal of virology2005
The 41-amino-acid C-terminal region of the hepatitis E virus ORF3 protein interacts with bikunin, a kunitz-type serine protease inhibitor.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Characteristics and Functions of HEV Proteins.Advances in experimental medicine and biology · 2023Article
- The Viral ORF3 Protein Is Required for Hepatitis E Virus Apical Release and Efficient Growth in Polarized Hepatocytes and Humanized Mice.Journal of virology · 2021Article
- Life cycle and morphogenesis of the hepatitis E virus.Emerging microbes & infections · 2018Review
- HEV ORF3 downregulates TLR7 to inhibit the generation of type I interferon via impairment of multiple signaling pathways.Scientific reports · 2018Article
- Hepatitis E virus: advances and challenges.Nature reviews. Gastroenterology & hepatology · 2018Review
- ORF3 as a sensitive and specific diagnostic index for hepatitis E.Experimental and therapeutic medicine · 2017Article
- Genotype-Specific Evolution of Hepatitis E Virus.Journal of virology · 2017Article
- Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins.Scientific reports · 2016Article
- Molecular Biology and Infection of Hepatitis E Virus.Frontiers in microbiology · 2016Review
- Article
- Therapeutic targets for the treatment of hepatitis E virus infection.Expert opinion on therapeutic targets · 2015Review
- Hepatitis e: molecular virology and pathogenesis.Journal of clinical and experimental hepatology · 2013Review
- Antigenic determinants of hepatitis E virus and vaccine-induced immunogenicity and efficacy.Journal of gastroenterology · 2013Review
- Virus host protein interaction network analysis reveals that the HEV ORF3 protein may interrupt the blood coagulation process.PloS one · 2013Article
- Molecular biology and replication of hepatitis E virus.Emerging microbes & infections · 2012Review
- The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release.Journal of virology · 2012Article
- Hepatitis E virus replication requires an active ubiquitin-proteasome system.Journal of virology · 2012Article
- Phylogenetic analysis of hepatitis e virus in northwest India.Hepatitis research and treatment · 2012Article
- Molecular virology of hepatitis E virus.Virus research · 2011Review
- Three amino acid mutations (F51L, T59A, and S390L) in the capsid protein of the hepatitis E virus collectively contribute to virus attenuation.Journal of virology · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Hepatitis E virus (HEV), a human plus-stranded RNA virus, contains three open reading frames (ORF). Of these, ORF1 encodes the viral nonstructural polyprotein, ORF2 encodes the major capsid protein, and ORF3 codes for a phosphoprotein of undefined function. Recently, using the yeast two-hybrid system to screen a human cDNA liver library, we have isolated and characterized AMBP (alpha1-microglobulin/bikunin precursor), which specifically interacts with the ORF3 protein of HEV. The ORF3 protein expedites the processing and secretion of alpha1-microglobulin. When checked individually for interaction, the second processed protein from AMBP, bikunin, strongly interacted with the full-length ORF3 protein. This protein-protein interaction has been validated by immunoprecipitation in both COS-1 and Huh7 cells and by His6 pull-down assays. In dual-labeling immunofluorescent staining, followed by fluorescence microscopy of transfected human liver cells, ORF3 colocalized with endogenously expressed bikunin. Finally, a 41-amino-acid C-terminal region of ORF3 has been found to be responsible for interacting with bikunin. The importance of this virus-host protein-protein interaction, with reference to the viral life cycle, has been discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.