ArticleMolecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology2005
Use of duplex PCR-CTPP methods for CYP2E1RsaI/IL-2 T-330G and IL-1B C-31T/TNF-A T-1031C polymorphisms.
Article in Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 10 citations in OpenAlex.
- Evaluation of the association between TNF-α-1031 T/C polymorphism with oral lichen planus disease.BMC oral health · 2024Article
- Identification of functional tumor necrosis factor-alpha promoter variants associated withWorld journal of gastroenterology · 2022Observational
- A new PCR method: one primer amplification of PCR-CTPP products.Molecular biotechnology · 2012Article
- Glutathione S-transferases T1 null genotype is associated with susceptibility to aristolochic acid nephropathy.International urology and nephrology · 2012Article
- Association between Interleukin-1B C-31T polymorphism and obesity in Japanese.Journal of epidemiology · 2009Article
- Eradication rate of Helicobacter pylori according to genotypes of CYP2C19, IL-1B, and TNF-A.International journal of medical sciences · 2006Article
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8 authors at 2 institutions in 1 country.
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Abstract
backgroundTwo duplex polymerase chain reaction (PCR) with confronting two-pair primer (PCR-CTPP) methods were designed for cytochrome P450 (CYP) 2E1 RsaI and interleukin (IL-2) T-330G, and for IL-1B C-31T and tumor necrosis factor-alpha (TNF-A) T-1031C. The four polymorphisms are considered to be functional, and the three cytokines reportedly inhibit CYP2E1 expression. Many studies have reported associations between the above polymorphisms and risk of diseases including cancers and inflammatory diseases.
aimThe main objective of this study was to examine the applicability of the established PCR conditions to a real situation.
participantsParticipants were female examinees aged from 35 to 85 years who attended health checks run by a local government in Japan.
resultsThe allele frequencies among 325 female health check examinees were 0.804 for CYP2E1 c1 allele, 0.668 for IL-2-330T allele, 0.554 for IL-1B-31T allele, and 0.822 for TNF-A-1031T allele. p-Values from a Hardy-Weinberg equilibrium test were 0.658, 0.955, 0.062, and 0.806, respectively. DISCUSSION: Clear DNA bands observed with electrophoresis allowed us to genotype the four polymorphisms. The genotype frequencies were within the Hardy-Weinberg equilibrium test proportions, though the p-value for IL-1B C-31T was marginal.
conclusionsBoth duplex PCR-CTPP methods may be useful tools for studies on the association between these polymorphisms and disease risk.
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