ArticlePlant cell reports2006
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 71 citations in OpenAlex.
- Optimization of somatic embryogenesis protocol for Hevea brasiliensis clones RRIM 600 and REYAN 88-13.Journal, genetic engineering & biotechnology · 2026Article
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- Micrografting Technique ofBio-protocol · 2025Article
- Overexpression ofInternational journal of molecular sciences · 2024Article
- Optimization of the Transformation Protocol for Increased Efficiency of Genetic Transformation inPlants (Basel, Switzerland) · 2022Article
- Biosynthesis of Natural Rubber: Current State and Perspectives.International journal of molecular sciences · 2018Review
- Overexpression of Hevea brasiliensis ethylene response factor HbERF-IXc5 enhances growth and tolerance to abiotic stress and affects laticifer differentiation.Plant biotechnology journal · 2018Article
- Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis).International journal of molecular sciences · 2016Article
- Gain-of-function inSpringerPlus · 2016Article
- Over-expression of a cytosolic isoform of the HbCuZnSOD gene in Hevea brasiliensis changes its response to a water deficit.Plant molecular biology · 2012Article
- Historical and recent achievements in the field of microbial degradation of natural and synthetic rubber.Applied and environmental microbiology · 2012Review
- Variation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants.Plant cell reports · 2011Article
- Agrobacterium rhizogenes-mediated transformation of Prunus as an alternative for gene functional analysis in hairy-roots and composite plants.Plant cell reports · 2011Article
- Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures.BMC plant biology · 2011Article
- The green fluorescent protein as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation in Hevea brasiliensis (Müll. Arg).Plant cell reports · 2010Article
- Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.).Plant cell reports · 2007Article
- LowIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA105 harbouring the vector pCAMBIA2301. Transformation capacity and competence of the embryogenic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20 degrees C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryogenesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.