ArticleBiological procedures online2005
Easy detection of chromatin binding proteins by the Histone Association Assay.
Article in Biological procedures online, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Dominant-negative variants in CBX1 cause a neurodevelopmental disorder.Genetics in medicine : official journal of the American College of Medical Genetics · 2023Article
- Single amino-acid mutation in a Drosoph ila melanogaster ribosomal protein: An insight in uL11 transcriptional activity.PloS one · 2022Article
- BRD4 methylation by the methyltransferase SETD6 regulates selective transcription to control mRNA translation.Science advances · 2021Article
- Prolonged Bat Call Exposure Induces a Broad Transcriptional Response in the Male Fall Armyworm (Frontiers in behavioral neuroscience · 2019Article
- Cancer-driving H3G34V/R/D mutations block H3K36 methylation and H3K36me3-MutSα interaction.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3.Nature structural & molecular biology · 2018Article
- In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- The pro-apoptotic BAX protein influences cell growth and differentiation from the nucleus in healthy interphasic cells.Cell cycle (Georgetown, Tex.) · 2017Article
- The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.PloS one · 2015Article
- Long noncoding RNA PANDA and scaffold-attachment-factor SAFA control senescence entry and exit.Nature communications · 2014Article
- Senescence is an endogenous trigger for microRNA-directed transcriptional gene silencing in human cells.Nature cell biology · 2012Article
- Recruitment of cAMP-response element-binding protein and histone deacetylase has opposite effects on glucocorticoid receptor gene transcription.The Journal of biological chemistry · 2010Article
- Enhanced gene trapping in mouse embryonic stem cells.Nucleic acids research · 2008Article
- Involvement of xeroderma pigmentosum group A (XPA) in progeria arising from defective maturation of prelamin A.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Yra1 is required for S phase entry and affects Dia2 binding to replication origins.Molecular and cellular biology · 2007Article
- Article
- Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast.Molecular and cellular biology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Histone Association Assay provides an easy approach for detecting proteins that bind chromatin in vivo. This technique is based on a chromatin immunoprecipitation protocol using histone H3-specific antibodies to precipitate bulk chromatin from crosslinked whole cell extracts. Proteins that co-precipitate with chromatin are subsequently detected by conventional SDS-PAGE and Western blot analysis. Unlike techniques that separate chromatin and non-chromatin interacting proteins by centrifugation, this method can be used to delineate whether a protein is chromatin associated regardless of its innate solubility. Moreover, the relative amount of protein bound to DNA can be ascertained under quantitative conditions. Therefore, this technique may be utilized for analyzing the chromatin association of proteins involved in diverse cellular processes.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.