ArticleMolecular and cellular biology2005
Multiple properties of the splicing repressor SRp38 distinguish it from typical SR proteins.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing.eLife · 2022Article
- SRSF10: an atypical splicing regulator with critical roles in stress response, organ development, and viral replication.RNA (New York, N.Y.) · 2021Review
- Identification of SRSF10 as a regulator of SMN2 ISS-N1.Human mutation · 2021Article
- Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis.Molecular cell · 2020Article
- Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.PLoS pathogens · 2020Article
- When SUMO met splicing.RNA biology · 2018Review
- SRSF10 Connects DNA Damage to the Alternative Splicing of Transcripts Encoding Apoptosis, Cell-Cycle Control, and DNA Repair Factors.Cell reports · 2016Article
- Mechanisms of heat shock response in mammals.Cellular and molecular life sciences : CMLS · 2013Review
- Review
- A single ancient origin for prototypical serine/arginine-rich splicing factors.Plant physiology · 2012Article
- Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator.Nature structural & molecular biology · 2008Article
- MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.Experimental cell research · 2007Article
- SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.Molecular and cellular biology · 2006Article
- Hsp27 enhances recovery of splicing as well as rephosphorylation of SRp38 after heat shock.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The SR protein SRp38 is a general splicing repressor that is activated by dephosphorylation during mitosis and in response to heat shock. Here we describe experiments that provide insights into the mechanism by which SRp38 functions in splicing repression. We first show that SRp38 redistributes and colocalizes with snRNPs, but not with a typical SR protein, SC35, during mitosis and following heat shock. Supporting the functional significance of this association, a micrococcal nuclease-sensitive component, i.e., an snRNP(s), completely rescued heat shock-induced splicing repression in vitro, and purified U1 snRNP did so partially. SRp38 contains an N-terminal RNA binding domain (RBD) and a C-terminal RS domain composed of two subdomains (RS1 and RS2 domains). Unexpectedly, an RS1 deletion mutant derivative specifically inhibited the second step of splicing, while an RS2 deletion mutant retained significant dephosphorylation-dependent repression activity. Using chimeric SRp38/SC35 proteins, we show that SC35-RBD/SRp38-RS can function as a general splicing activator and that the dephosphorylated version can act as a strong splicing repressor. SRp38-RBD/SC35-RS, however, was essentially inactive in these assays. Together, our results help to define the unusual features of SRp38 that distinguish it from other SR proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.