ArticleMolecular and cellular biology2005
PIAS1 activates the expression of smooth muscle cell differentiation marker genes by interacting with serum response factor and class I basic helix-loop-helix proteins.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- SWI/SNF Complex in Vascular Smooth Muscle Cells and Its Implications in Cardiovascular Pathologies.Cells · 2024Review
- TCF7L1 Accelerates Smooth Muscle Cell Phenotypic Switching and Aggravates Abdominal Aortic Aneurysms.JACC. Basic to translational science · 2023Article
- PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.PLoS pathogens · 2022Article
- Transcription factor 4 and its association with psychiatric disorders.Translational psychiatry · 2021Review
- Krüppel-like factors: Crippling and un-crippling metabolic pathways.JACC. Basic to translational science · 2018Article
- Krüppel-like factors in mammalian stem cells and development.Development (Cambridge, England) · 2017Review
- Regulation of vascular smooth muscle phenotype by cross-regulation of krüppel-like factors.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2017Article
- Myocardin in biology and disease.Journal of biomedical research · 2015Review
- Cell division cycle 7 mediates transforming growth factor-β-induced smooth muscle maturation through activation of myocardin gene transcription.The Journal of biological chemistry · 2013Article
- Protein inhibitor of activated STAT 1 (PIAS1) is identified as the SUMO E3 ligase of CCAAT/enhancer-binding protein β (C/EBPβ) during adipogenesis.Molecular and cellular biology · 2013Article
- Tcf15 primes pluripotent cells for differentiation.Cell reports · 2013Article
- Cooperative binding of KLF4, pELK-1, and HDAC2 to a G/C repressor element in the SM22α promoter mediates transcriptional silencing during SMC phenotypic switching in vivo.Circulation research · 2012Article
- Yap1 protein regulates vascular smooth muscle cell phenotypic switch by interaction with myocardin.The Journal of biological chemistry · 2012Article
- Cell division cycle 7 is a novel regulator of transforming growth factor-β-induced smooth muscle cell differentiation.The Journal of biological chemistry · 2012Article
- Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.PloS one · 2012Article
- Response gene to complement 32 is essential for fibroblast activation in renal fibrosis.The Journal of biological chemistry · 2011Article
- Smooth muscle cell differentiation in vitro: models and underlying molecular mechanisms.Arteriosclerosis, thrombosis, and vascular biology · 2011Review
- Smad3-mediated myocardin silencing: a novel mechanism governing the initiation of smooth muscle differentiation.The Journal of biological chemistry · 2011Article
- Novel role and mechanism of protein inhibitor of activated STAT1 in spatial learning.The EMBO journal · 2011Article
- Molecular regulation of contractile smooth muscle cell phenotype: implications for vascular tissue engineering.Tissue engineering. Part B, Reviews · 2010Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Although a critical component of vascular disease is modulation of the differentiated state of vascular smooth muscle cells (SMC), the mechanisms governing SMC differentiation are relatively poorly understood. We have previously shown that E-boxes and the ubiquitously expressed class I basic helix-loop-helix (bHLH) proteins, including E2-2 and E12, are important in regulation of the SMC differentiation marker gene, the SM alpha-actin gene. The aim of the present study was to identify proteins that bind to class I bHLH proteins in SMC and modulate transcriptional regulation of SMC differentiation marker genes. Herein we report that members of the protein inhibitor of activated STAT (PIAS) family interact with class I bHLH factors as well as serum response factor (SRF). PIAS1 interacted with E2-2 and E12 based on yeast two-hybrid screens, mammalian two-hybrid assays, and/or coimmunoprecipitation assays. Overexpression of PIAS1 significantly activated the SM alpha-actin promoter and mRNA expression, as well as SM myosin heavy chain and SM22alpha, whereas a small interfering RNA for PIAS1 decreased activity of these promoters, as well as endogenous mRNA expression, and SRF binding to SM alpha-actin promoter within intact chromatin in cultured SMC. Of significance, PIAS1 bound to SRF and activated SM alpha-actin promoter expression in wild-type but not SRF(-/-) embryonic stem cells. These results provide novel evidence that PIAS1 modulates transcriptional activation of SMC marker genes through cooperative interactions with both SRF and class I bHLH proteins.
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