ArticleJournal of cancer research and clinical oncology2005
The gut fermentation product butyrate, a chemopreventive agent, suppresses glutathione S-transferase theta (hGSTT1) and cell growth more in human colon adenoma (LT97) than tumor (HT29) cells.
Article in Journal of cancer research and clinical oncology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- A Model of Butyrate Activity and Resistance in CRC.Journal of cellular and molecular medicine · 2025Review
- Review
- Article
- Short-chain fatty acids reprogram metabolic profiles with the induction of reactive oxygen species production in human colorectal adenocarcinoma cells.Computational and structural biotechnology journal · 2023Article
- Oncogenic and Receptor-Mediated Wnt Signaling Influence the Sensitivity of Colonic Cells to Butyrate.Journal of Cancer · 2023Article
- Use of the β-Glucan-Producing Lactic Acid Bacteria StrainsNutrients · 2022Article
- Hypothesis: Sam68 and Pygo2 mediate cell type-specific effects of the modulation of CBP-Wnt and p300-Wnt activities in Colorectal Cancer Cells.Journal of Cancer · 2021Article
- Hypothesis: Retinoblastoma protein inactivation mediates effects of histone deacetylase inhibitor-induced Wnt hyperactivation in colorectal cancer cells.Journal of Cancer · 2020Article
- Hypothesis: Induction of biomarkers for detection of colonic neoplasms.Journal of Cancer · 2018Article
- ZEB1 Mediates Drug Resistance and EMT in p300-Deficient CRC.Journal of Cancer · 2017Article
- Vimentin, colon cancer progression and resistance to butyrate and other HDACis.Journal of cellular and molecular medicine · 2016Review
- Determination of the Role of CBP- and p300-Mediated Wnt Signaling on Colonic Cells.JMIR research protocols · 2016Article
- Influence of miRNA-106b and miRNA-135a on butyrate-regulated expression of p21 and Cyclin D2 in human colon adenoma cells.Genes & nutrition · 2015Article
- CREB-binding protein, p300, butyrate, and Wnt signaling in colorectal cancer.World journal of gastroenterology · 2015Review
- Butyrate induced changes in Wnt-signaling specific gene expression in colorectal cancer cells.BMC research notes · 2014Article
- Modulation of Wnt Activity and Cell Physiology by Butyrate in LT97 Microadenoma Cells.Journal of Cancer · 2014Article
- Article
- CBP Activity Mediates Effects of the Histone Deacetylase Inhibitor Butyrate on WNT Activity and Apoptosis in Colon Cancer Cells.Journal of Cancer · 2013Article
- p300 Influences Butyrate-Mediated WNT Hyperactivation In Colorectal Cancer Cells.Journal of Cancer · 2013Article
- Chemopreventive effects of in vitro digested and fermented bread in human colon cells.European journal of nutrition · 2012Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe gut fermentation product of dietary fiber, butyrate, inhibits growth of HT29, an established tumor cell line. It also induces detoxifying enzymes belonging to the glutathione S-transferase family (GSTs), namely hGSTM2, hGSTP1, hGSTA4, but not of hGSTT1 . Here we investigated kinetics of effects in HT29 and compared sensitivities with preneoplastic LT97 colon adenoma cells, to assess mechanisms of colon cancer chemoprevention in two stages of cell transformation.
methodsWe determined cell growth after butyrate treatment by quantifying DNA, GST expression by Northern/Western Blotting or biochemical analysis and butyrate consumption by measuring the residual concentrations in the cell culture supernatants. Stability of GST-theta (hGSTT1) mRNA was assessed in HT29 cells after inhibition of transcription with actinomycin D.
resultsLT97 adenoma cells consumed twofold more butyrate and were more sensitive to growth inhibition than HT29 (EC(50)1.9 mM and 4.0 mM, respectively). Butyrate did not induce GSTs, but instead reduced hGSTT1 in LT97 and HT29.
conclusionsButyrate has suppressing-agent activities in human colon cells by inhibiting two survival factors, namely hGSTT1 and cell growth, with LT97 more sensitive than HT29. These findings indicate that butyrate formation in the gut lumen of humans could be protective by reducing survival of transformed colon cells.
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