ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
SuperSAGE combined with PCR walking allows global gene expression profiling of banana (Musa acuminata), a non-model organism.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa.BMC genomics · 2009Pooled it
- Light dependent protochlorophyllide oxidoreductase: a succinct look.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- The quest for tolerant varieties: the importance of integrating "omics" techniques to phenotyping.Frontiers in plant science · 2015Review
- Assessment of RNAi-induced silencing in banana (Musa spp.).BMC research notes · 2014Article
- Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa.Molecular breeding : new strategies in plant improvement · 2012Article
- In silico identification of known osmotic stress responsive genes from Arabidopsis in soybean and Medicago.Genetics and molecular biology · 2012Article
- New insights in the sugarcane transcriptome responding to drought stress as revealed by superSAGE.TheScientificWorldJournal · 2012Article
- The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE.BMC plant biology · 2011Article
- Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish.BMC genomics · 2010Article
- SuperSAGE: the drought stress-responsive transcriptome of chickpea roots.BMC genomics · 2008Article
- Analysis of wheat SAGE tags reveals evidence for widespread antisense transcription.BMC genomics · 2008Article
- Domestication, genomics and the future for banana.Annals of botany · 2007Review
- Serial analysis of gene expression in sugarcane (Saccharum spp.) leaves revealed alternative C4 metabolism and putative antisense transcripts.Plant molecular biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-serial analysis of gene expression (SuperSAGE) was used to characterize, for the first time, the global gene expression pattern in banana (Musa acuminata). A total of 10,196 tags were generated from leaf tissue, representing 5,292 expressed genes. Forty-nine tags of the top 100 most abundantly expressed transcripts were annotated by homology to cDNA or EST sequences. Typically for leaf tissue, analysis of the transcript profiles showed that the majority of the abundant transcripts are involved in energy production, mainly photosynthesis. However, the most abundant tag was derived from a type 3 metallothionein transcript, which accounted for nearly 3% of total transcripts analysed. Furthermore, the 26-bp long SuperSAGE tags were applied in 3'-rapid amplification of cDNA ends (3'RACE) for the identification of unknown tags. In combination with thermal asymmetric interlaced PCR (TAIL-PCR), this allowed the recovery of a full gene sequence of a novel NADPH:protochlorophyllide oxidoreductase, the key enzyme in chlorophyll biosynthesis. SuperSAGE in conjunction with 3'RACE and TAIL-PCR will be a powerful tool for transcriptomics of non-model, but otherwise important organisms.
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