ArticleMycorrhiza2005
Agrobacterium-mediated transformation of the ectomycorrhizal symbiont Laccaria bicolor S238N.
Article in Mycorrhiza, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Laccaria bicolor adapts to phosphate deficiency at the developmental, transcriptional and metabolic levels.Mycorrhiza · 2025Article
- Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides.The New phytologist · 2022Article
- Genes and evolutionary fates of the amanitin biosynthesis pathway in poisonous mushrooms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genetic evidence for the requirements of antroquinonol biosynthesis by Antrodia camphorata during liquid-state fermentation.Journal of industrial microbiology & biotechnology · 2022Article
- Article
- The development of an efficient RNAi system based on Agrobacterium-mediated transformation approach for studying functional genomics in medical fungus Wolfiporia cocos.World journal of microbiology & biotechnology · 2020Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Agrobacterium-mediated insertional mutagenesis in the mycorrhizal fungus Laccaria bicolor.Current genetics · 2017Article
- The good, the bad and the tasty: The many roles of mushrooms.Studies in mycology · 2016Article
- Model systems to unravel the molecular mechanisms of heavy metal tolerance in the ericoid mycorrhizal symbiosis.Mycorrhiza · 2016Review
- Expanding genomics of mycorrhizal symbiosis.Frontiers in microbiology · 2014Review
- From soil to plant, the journey of P through trophic relationships and ectomycorrhizal association.Frontiers in plant science · 2014Review
- Bacterial-fungal interactions: hyphens between agricultural, clinical, environmental, and food microbiologists.Microbiology and molecular biology reviews : MMBR · 2011Review
- pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA - triggering in the mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2010Article
- Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus.Applied and environmental microbiology · 2009Article
- Investigating dominant selection markers for Coprinopsis cinerea: a carboxin resistance system and re-evaluation of hygromycin and phleomycin resistance vectors.Current genetics · 2009Article
- The dominant Hc.Sdh (R) carboxin-resistance gene of the ectomycorrhizal fungus Hebeloma cylindrosporum as a selectable marker for transformation.Current genetics · 2009Article
- T-DNA insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2008Article
- Imaging mycorrhizal fungal transformants that express EGFP during ericoid endosymbiosis.Current genetics · 2007Article
- Gene knockdown by ihpRNA-triggering in the ectomycorrhizal basidiomycete fungus Laccaria bicolor.Bioengineered bugsArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of an efficient transformation system is required to alter the expression of symbiosis-regulated genes and to develop insertional mutagenesis in the ectomycorrhizal basidiomycete Laccaria bicolor S238N. Vegetative mycelium of this fungus was transformed by Agrobacterium tumefaciens-mediated gene transfer. The selection marker was the hygromycin resistance gene of Escherichia coli (hph) under the control of the gpd promoter from Agaricus bisporus and the CaMV 35S terminator as part of the T-DNA. PCR amplification of hph and Southern blot analyses showed that the genome of the hygromycin-resistant transformants contained the cassette. The latter proved mostly single copy and random integration of part of the transgene into the fungal genome. A. tumefaciens-mediated gene transfer should facilitate future development of insertional mutagenesis, targeted gene disruption and RNA interference technology in L. bicolor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.