ArticleHistochemistry and cell biology2005
Nuclear distribution of actin and myosin I depends on transcriptional activity of the cell.
Article in Histochemistry and cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- Indirect visualization of endogenous nuclear actin by correlative light and electron microscopy (CLEM) using an actin-directed chromobody.Histochemistry and cell biology · 2019Article
- Nuclear Actin: From Discovery to Function.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Calcium and Nuclear Signaling in Prostate Cancer.International journal of molecular sciences · 2018Review
- Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins.Nucleus (Austin, Tex.) · 2018Article
- Myosin-I molecular motors at a glance.Journal of cell science · 2016Review
- The Histochemistry and Cell Biology omnium-gatherum: the year 2015 in review.Histochemistry and cell biology · 2016Review
- β- and γ-Actins in the nucleus of human melanoma A375 cells.Histochemistry and cell biology · 2015Article
- Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation.BMC biology · 2015Article
- The potential roles of actin in the nucleus.Cell journal · 2015Review
- Simultaneous detection of multiple targets for ultrastructural immunocytochemistry.Histochemistry and cell biology · 2014Article
- Mouse nuclear myosin I knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus.PloS one · 2013Article
- 3D chromatin conformation correlates with replication timing and is conserved in resting cells.Nucleic acids research · 2012Article
- Nuclear actin and lamins in viral infections.Viruses · 2012Review
- Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner.PloS one · 2012Article
- Nuclear actin and myosins: life without filaments.Nature cell biology · 2011Review
- Differential sublocalization of actin variants within the nucleus.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus.Histochemistry and cell biology · 2010Article
- Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei.Molecular & cellular proteomics : MCP · 2010Article
- Actin complexes in the cell nucleus: new stones in an old field.Histochemistry and cell biology · 2010Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As previous studies suggested, nuclear myosin I (NMI) and actin have important roles in DNA transcription. In this study, we characterized the dynamics of these two proteins during transcriptional activation in phytohemagglutinin (PHA) stimulated human lymphocytes. The stimulation led to strong up-regulation of NMI both on the mRNA and protein level, while actin was relatively stably expressed. The intranuclear distribution of actin and NMI was evaluated using immunogold labeling. In nucleoli of resting cells, actin was localized predominantly to fibrillar centers (FCs), while NMI was located mainly to the dense fibrillar component (DFC). Upon stimulation, FCs remained the main site of actin localization, however, an accumulation of both actin and NMI in the DFC and in the granular component was observed. In the nucleoplasm of resting lymphocytes, both actin and NMI were localized mostly in condensed chromatin. Following stimulation, the majority of both proteins shifted towards the decondensed chromatin. In transcriptionally active cells, both actin and NMI colocalized with nucleoplasmic transcription sites. These results demonstrate that actin and NMI are compartmentalized in the nuclei where they can dynamically translocate depending on transcriptional activity of the cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.