ArticleCurrent microbiology2005
Chitinase production in solid-state fermentation by Enterobacter sp. NRG4 using statistical experimental design.
Article in Current microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- Enhanced production of chitinase from Bacillus cereus V5331 and evaluation of anti-candidal potential of enzyme: central composite design approach.Antonie van Leeuwenhoek · 2026Article
- Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Article
- Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products.Marine drugs · 2020Review
- Chitinases from Bacteria to Human: Properties, Applications, and Future Perspectives.Enzyme research · 2015Review
- Optimization of biodegradable plastic production on sugar cane molasses in Enterobacter sp. SEL2.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Chitinases: An update.Journal of pharmacy & bioallied sciences · 2013Article
- Biodegradation of shrimp processing bio-waste and concomitant production of chitinase enzyme and N-acetyl-D-glucosamine by marine bacteria: production and process optimization.World journal of microbiology & biotechnology · 2012Article
- Chitinase production in solid-state fermentation from Oerskovia xanthineolytica NCIM 2839 and its application in fungal protoplasts formation.Current microbiology · 2011Article
- Production of N-acetylglucosamine using recombinant chitinolytic enzymes.Indian journal of microbiology · 2011Article
- Thermoactive β-N-acetylhexosaminidase production by a soil isolate of Penicillium monoverticillium CFR 2 under solid state fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin.World journal of microbiology & biotechnology · 2011Article
- Chitinolyticbacter meiyuanensis SYBC-H1T, gen. nov., sp. nov., a chitin-degrading bacterium isolated from soil.Current microbiology · 2011Article
- Acid protease production by solid-state fermentation using Aspergillus oryzae MTCC 5341: optimization of process parameters.Journal of industrial microbiology & biotechnology · 2010Article
- Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology.Journal of Zhejiang University. Science. B · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The optimization of nutrient levels for chitinase production by Enterobacter sp. NRG4 in solid-state fermentation conditions (SSF) was carried out using response surface methodology (RSM) based on central composite design (CCD). The design was employed by selecting wheat bran-to-flake chitin ratio, moisture level, inoculum size, and incubation time as model factors. The results of first-order factorial design experiments showed that all four independent variables have significant effects on chitinase production. The optimum concentrations for chitinase production were wheat bran-to-flake chitin ratio, 1; moisture level, 80%; inoculum size, 2.6 mL; and incubation time, 168 h. Using this statistical optimization method, chitinase production was found to increase from 616 U . g(-1) dry weight of solid substrate to 1475 U . g(-1) dry weight of solid substrate.
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