ArticleProtein science : a publication of the Protein Society2005
Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.
Article in Protein science : a publication of the Protein Society, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The Myotubularin Related Proteins and the Untapped Interaction Potential of Their Disordered C-Terminal Regions.Proteins · 2025Article
- Redox Regulation of KAntioxidants & redox signaling · 2024Review
- NMR Studies of the Interactions between Sialyllactoses and the Polysialytransferase Domain for Polysialylation Inhibition.Current issues in molecular biology · 2024Article
- The Bifunctional Effects of Lactoferrin (LFcinB11) in Inhibiting Neural Cell Adhesive Molecule (NCAM) Polysialylation and the Release of Neutrophil Extracellular Traps (NETs).International journal of molecular sciences · 2024Article
- Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport.Nature structural & molecular biology · 2024Article
- Structural characterization of KKT4, an unconventional microtubule-binding kinetochore protein.Structure (London, England : 1993) · 2021Article
- Molecular Interactions of the Polysialytransferase Domain (PSTD) in ST8Sia IV with CMP-Sialic Acid and Polysialic Acid Required for Polysialylation of the Neural Cell Adhesion Molecule Proteins: An NMR Study.International journal of molecular sciences · 2020Article
- Proof of Principle for a Novel Class of Antihypertensives That Target the Oxidative Activation of PKG Iα (Protein Kinase G Iα).Hypertension (Dallas, Tex. : 1979) · 2017Article
- NMR insight into myosin-binding subunit coiled-coil structure reveals binding interface with protein kinase G-Iα leucine zipper in vascular function.The Journal of biological chemistry · 2017Article
- Structures of cGMP-Dependent Protein Kinase (PKG) Iα Leucine Zippers Reveal an Interchain Disulfide Bond Important for Dimer Stability.Biochemistry · 2015Article
- Crystal structure of the cGMP-dependent protein kinase II leucine zipper and Rab11b protein complex reveals molecular details of G-kinase-specific interactions.The Journal of biological chemistry · 2014Article
- iNR-Drug: predicting the interaction of drugs with nuclear receptors in cellular networking.International journal of molecular sciences · 2014Article
- The disposition of the LZCC protein residues in wenxiang diagram provides new insights into the protein-protein interaction mechanism.Journal of theoretical biology · 2011Article
- An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics.The Journal of biological chemistry · 2011Article
- Oxidant sensing by protein kinases a and g enables integration of cell redox state with phosphoregulation.Sensors (Basel, Switzerland) · 2010Review
- Transnitrosylating nitric oxide species directly activate type I protein kinase A, providing a novel adenylate cyclase-independent cross-talk to beta-adrenergic-like signaling.The Journal of biological chemistry · 2009Article
- A Two-amino Acid Mutation Encountered in Duchenne Muscular Dystrophy Decreases Stability of the Rod Domain 23 (R23) Spectrin-like Repeat of Dystrophin.The Journal of biological chemistry · 2009Article
- Probing the interaction between the coiled coil leucine zipper of cGMP-dependent protein kinase Ialpha and the C terminus of the myosin binding subunit of the myosin light chain phosphatase.The Journal of biological chemistry · 2008Article
- Assignment-free solution NMR method reveals CesT as an unswapped homodimer.Protein science : a publication of the Protein Society · 2008Article
- Estimating residue evolutionary conservation by introducing von Neumann entropy and a novel gap-treating approach.Amino acids · 2008Article
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5 authors.
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Abstract
Coiled-coil motifs play essential roles in protein assembly and molecular recognition, and are therefore the targets of many ongoing structural and functional studies. However, owing to the dynamic nature of many of the smaller coiled-coil domains, crystallization for X-ray studies is very challenging. Determination of elongated structures using standard NMR approaches is inefficient and usually yields low-resolution structures due to accumulation of small errors over long distances. Here we describe a solution NMR approach based on residual dipolar couplings (RDCs) for rapid and accurate structure determination of coiled-coil dimers. Using this approach, we were able to determine the high-resolution structure of the coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of previously unknown structure that is critical for physiological relaxation of vascular smooth muscle. This approach can be extended to solve coiled-coil structures with higher order assemblies.
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