ArticleAmerican journal of physiology. Renal physiology2006
Characterization of ammonia transport by the kidney Rh glycoproteins RhBG and RhCG.
Article in American journal of physiology. Renal physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 118 citations in OpenAlex.
- Hyperuricemia increases susceptibility to chronic kidney injury exacerbation via autophagic flux blockade-mediated ammonia death pathway.Journal of advanced research · 2026Article
- Mutations in ammonia transporter RhBG that impair NHThe Journal of physiology · 2026Article
- Prognostic and immunological roles of ammonia-induced cell death-related genes in non-small cell lung cancer.BMC pulmonary medicine · 2026Article
- Comprehensive analysis of ammonia-induced cell death and GLS1 in gastric adenocarcinoma: implications for prognosis and therapeutic strategies.Cancer cell international · 2025Article
- Rhbg interaction with CA-IV and its effects on NHAmerican journal of physiology. Cell physiology · 2025Article
- Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8Nature cell biology · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- Metabolic acidosis and hyperkalemia differentially regulate cation HCN3 channel in the rat nephron.Journal of molecular histology · 2020Article
- Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.Nature communications · 2020Article
- Effects of chronic hypercapnia on ammonium transport in the mouse kidney.Physiological reports · 2019Article
- Active mode of excretion across digestive tissues predates the origin of excretory organs.PLoS biology · 2019Article
- Ethanol sensitizes skeletal muscle to ammonia-induced molecular perturbations.The Journal of biological chemistry · 2019Article
- Differences in renal ammonia metabolism in male and female kidney.American journal of physiology. Renal physiology · 2018Article
- Proximal tubule glutamine synthetase expression is necessary for the normal response to dietary protein restriction.American journal of physiology. Renal physiology · 2017Article
- Ammonia Transporters and Their Role in Acid-Base Balance.Physiological reviews · 2017Review
- Structural determinants of NH3 and NH4+ transport by mouse Rhbg, a renal Rh glycoprotein.American journal of physiology. Renal physiology · 2016Article
- Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins.American journal of physiology. Cell physiology · 2015Article
- NBCe1 expression is required for normal renal ammonia metabolism.American journal of physiology. Renal physiology · 2015Article
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Effect of dietary protein restriction on renal ammonia metabolism.American journal of physiology. Renal physiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The erythrocyte Rh-associated glycoprotein (RhAG) was recently found to mediate transport of ammonia/ammonium when expressed in Xenopus laevis oocytes and yeast Saccharomyces cerevisiae. Nonerythroid homologs, RhBG and RhCG, are expressed in the mammalian kidney connecting segment and the collecting duct, major sites of urinary ammonia secretion. This study characterizes the transport properties of murine RhBG and RhCG by ammonium analog [14C]methylamine (MA) uptake and two-electrode voltage clamping of X. laevis oocytes. Both RhBG and RhCG mediated transport of ammonia, but differed in affinity for substrate (K(m) = 2.5 and 10 mM, respectively). The rates of RhBG- and RhCG-mediated transport were sensitive to the concentration of the protonated MA species and were stimulated by extracellular alkalosis and inhibited by acidosis, suggesting a role for H+ in the transport process. Whereas expression of RhBG or RhCG caused a small increase in plasma membrane conductance, [14C]MA uptake was not affected by depolarization of oocytes with 100 mM extracellular K+ or by clamping the membrane potential between 0 and -100 mV, indicating that RhBG- and RhCG-mediated transport was independent of the membrane potential. These results strongly suggest that RhBG and RhCG transport ammonia by an electroneutral process that involves NH4(+)/H+ exchange resulting in net NH3 translocation. The polarized localization of RhBG and RhCG in kidney tubules and the different substrate affinities may enable these proteins to participate in transepithelial ammonia secretion and to therefore play an important role in whole animal acid-base regulation.
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