ArticleRNA (New York, N.Y.)2005
Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Pushing the Limits of Nucleic Acid Function.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Review
- Composite Hydrogel Microspheres Encapsulating Hollow Mesoporous Imprinted Nanoparticles for Selective Capture and Separation of 2'-Deoxyadenosine.Molecules (Basel, Switzerland) · 2022Article
- Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey.Angewandte Chemie (International ed. in English) · 2021Review
- A GTP-synthesizing ribozyme selected by metabolic coupling to an RNA polymerase ribozyme.Science advances · 2021Article
- Directed Evolution Methods for Enzyme Engineering.Molecules (Basel, Switzerland) · 2021Review
- Water/Oil Emulsions with Controlled Droplet Sizes forACS omega · 2021Article
- High-throughput screening of biomolecules using cell-free gene expression systems.Synthetic biology (Oxford, England) · 2018Review
- An in vitro compartmentalization-based method for the selection of bond-forming enzymes from large libraries.Biotechnology and bioengineering · 2016Article
- Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection.Molecules (Basel, Switzerland) · 2015Review
- A high-throughput screen for antibiotic drug discovery.Biotechnology and bioengineering · 2014 · on this mapArticle
- Directed evolution of genetic parts and circuits by compartmentalized partnered replication.Nature biotechnology · 2014Article
- RNA catalysis through compartmentalization.Nature chemistry · 2012Article
- Cell-free synthetic biology: thinking outside the cell.Metabolic engineering · 2012Review
- A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System.Journal of nucleic acids · 2012Article
- DNA as a versatile chemical component for catalysis, encoding, and stereocontrol.Angewandte Chemie (International ed. in English) · 2010Review
- Microfluidic compartmentalized directed evolution.Chemistry & biology · 2010Article
- A class I ligase ribozyme with reduced Mg2+ dependence: Selection, sequence analysis, and identification of functional tertiary interactions.RNA (New York, N.Y.) · 2009Article
- Man versus machine versus ribozyme.PLoS biology · 2008Article
- Selection of an improved RNA polymerase ribozyme with superior extension and fidelity.RNA (New York, N.Y.) · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.