ArticleAntimicrobial agents and chemotherapy2005
Structure-function characterization and optimization of a plant-derived antibacterial peptide.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Mechanisms, Modulation, and Mitigation: Dietary-Gut Microbiome Strategies Against Antibiotic Resistance.Probiotics and antimicrobial proteins · 2026Review
- Peptides from General By-Products: Unveiling Their Potential Biological Activities in Human Health.Molecules (Basel, Switzerland) · 2025Review
- Antimicrobial properties of tomato juice and peptides against typhoidalMicrobiology spectrum · 2024Article
- Antibacterial and Antiviral Properties of Chenopodin-Derived Synthetic Peptides.Antibiotics (Basel, Switzerland) · 2024Article
- Antibacterial effect ofOpen veterinary journal · 2024Article
- Antimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata.World journal of microbiology & biotechnology · 2023Article
- Pneumonia-associated microbial species and stabilized chlorine dioxideJADA foundational science · 2023Article
- Moringa oleifera-derived coagulants for water treatment: Floc structure, residual organics, and performance trade-offs.Environmental science and pollution research international · 2022Article
- Antioxidant, Antibacterial Activities and Mineral Content of Buffalo Yoghurt Fortified with Fenugreek andFoods (Basel, Switzerland) · 2020Article
- Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance.Antibiotics (Basel, Switzerland) · 2020Review
- Article
- Histological Assessment of Palatal Donor Site Wound Healing after Application of Moringa oleifera Lamarck Leaf Extract in Rats.European journal of dentistry · 2019Article
- Plant and Microalgae Derived Peptides Are Advantageously Employed as Bioactive Compounds in Cosmetics.Frontiers in plant science · 2019Review
- Insect Antimicrobial Peptides, a Mini Review.Toxins · 2018Review
- Microbiological Assessment ofOpen access Macedonian journal of medical sciences · 2016Article
- Characterization and antimicrobial activity of 4-(β-D-glucopyranosyl-1→4-α-L-rhamnopyranosyloxy)-benzyl thiocarboxamide; a novel bioactive compound from Moringa oleifera seed extract.Folia microbiologica · 2010Article
- Anti-cyanobacterial activity of Moringa oleifera seeds.Journal of applied phycology · 2010Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crushed seeds of the Moringa oleifera tree have been used traditionally as natural flocculants to clarify drinking water. We previously showed that one of the seed peptides mediates both the sedimentation of suspended particles such as bacterial cells and a direct bactericidal activity, raising the possibility that the two activities might be related. In this study, the conformational modeling of the peptide was coupled to a functional analysis of synthetic derivatives. This indicated that partly overlapping structural determinants mediate the sedimentation and antibacterial activities. Sedimentation requires a positively charged, glutamine-rich portion of the peptide that aggregates bacterial cells. The bactericidal activity was localized to a sequence prone to form a helix-loop-helix structural motif. Amino acid substitution showed that the bactericidal activity requires hydrophobic proline residues within the protruding loop. Vital dye staining indicated that treatment with peptides containing this motif results in bacterial membrane damage. Assembly of multiple copies of this structural motif into a branched peptide enhanced antibacterial activity, since low concentrations effectively kill bacteria such as Pseudomonas aeruginosa and Streptococcus pyogenes without displaying a toxic effect on human red blood cells. This study thus identifies a synthetic peptide with potent antibacterial activity against specific human pathogens. It also suggests partly distinct molecular mechanisms for each activity. Sedimentation may result from coupled flocculation and coagulation effects, while the bactericidal activity would require bacterial membrane destabilization by a hydrophobic loop.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.