ArticleProceedings of the National Academy of Sciences of the United States of America2005
Gliding ghosts of Mycoplasma mobile.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 103 citations in OpenAlex.
- Gliding direction ofBiophysics and physicobiology · 2025Article
- Innovative Methodology for Antimicrobial Susceptibility Determination inMicroorganisms · 2024Article
- A tradeoff between bacteriophage resistance and bacterial motility is mediated by the Rcs phosphorelay inMicrobiology (Reading, England) · 2024Article
- A geometrical theory of gliding motility based on cell shape and surface flow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Internal structure ofBiophysics and physicobiology · 2024Article
- Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cell shape controls rheotaxis in small parasitic bacteria.PLoS pathogens · 2022Article
- High-speed Atomic Force Microscopy Observation of Internal Structure Movements in LivingBio-protocol · 2022Article
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- Chained Structure of Dimeric FmBio · 2021Article
- Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy.mBio · 2021Article
- Prospects for the Mechanism ofFrontiers in microbiology · 2021Review
- Motile ghosts of the halophilic archaeon,Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Coexistence of Two Chiral Helices Produces Kink Translation inJournal of bacteriology · 2020Article
- Identification and sequence analyses of the gliding machinery proteins from Mycoplasma mobile.Scientific reports · 2020Article
- Article
- Behaviors and Energy Source ofJournal of bacteriology · 2019Article
- Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding.FEMS microbiology letters · 2019Article
- Motor torque measurement ofCommunications biology · 2019Article
- Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.Scientific reports · 2018Article
Corrections and comments
- Commented on by
- Erratum issued
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several species of mycoplasmas glide on solid surfaces, in the direction of their membrane protrusion at a cell pole, by an unknown mechanism. Our recent studies on the fastest species, Mycoplasma mobile, suggested that the gliding machinery, localized at the base of the membrane protrusion (the "neck"), is composed of two huge proteins. This machinery forms spikes sticking out from the neck and propels the cell by alternately binding and unbinding the spikes to a solid surface. Here, to study the intracellular mechanisms for gliding, we established a permeabilized gliding ghost model, analogous to the "Triton model" of the eukaryotic axoneme. Treatment with Triton X-100 stopped the gliding and converted the cells to permeabilized "ghosts." When ATP was added exogenously, approximately 85% of the ghosts were reactivated, gliding at speeds similar to those of living cells. The reactivation activity and inhibition by various nucleotides and ATP analogs, as well as their kinetic parameters, showed that the machinery is driven by the hydrolysis of ATP to ADP plus phosphate, caused by an unknown ATPase.
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