ArticlePlant physiology2005
Cassava plants with a depleted cyanogenic glucoside content in leaves and tubers. Distribution of cyanogenic glucosides, their site of synthesis and transport, and blockage of the biosynthesis by RNA interference technology.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 269 citations in OpenAlex.
- Metabolic Enzyme MeHNL11 Regulates MeCAS1b Transcription for Cyanide Reutilization in Response to Nitrate Deficiency in Cassava.Plant biotechnology journal · 2026Article
- Functional insights into dispensable genes using genome-wide loss-of-function burden tests in Arabidopsis.The Plant cell · 2026Article
- Genomic prediction accuracy for low hydrogen cyanide selection in fresh cassava roots. Comparative model analysis.BMC plant biology · 2026Article
- Transport of secondary metabolites in plants: Mechanistic insights and transporter engineering for crop improvement.Plant communications · 2025Review
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- Dhurrin: a potential endogenous nitrogen turnover source for early seedling growth in sorghum.Frontiers in plant science · 2025Article
- Genetic Variation and Heritability for Hydrogen Cyanide in Fresh Cassava Roots: Implications for Low-Cyanide Cassava Breeding.Plants (Basel, Switzerland) · 2024Article
- The Putative GATA Transcription FactorLife (Basel, Switzerland) · 2024Article
- Transporter editing in cassava indicates local production of cyanogenic glucosides in, and export from, cassava roots.Plant biotechnology journal · 2024Article
- Correlate the cyanogenic potential and dry matter content of cassava roots and leaves grown in different environments.Scientific reports · 2023Article
- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Diverse organ-specific localisation of a chemical defence, cyanogenic glycosides, in flowers of eleven species of Proteaceae.PloS one · 2023Article
- Engineering medicinal plant-derived CYPs: a promising strategy for production of high-valued secondary metabolites.Planta · 2022Review
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- CRISPR-Cas9-mediated knockout ofFrontiers in plant science · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Targeted mutagenesis of theFrontiers in plant science · 2022Article
- A cassava common mosaic virus vector for virus-induced gene silencing in cassava.Plant methods · 2021Article
- Genetics and breeding for climate change in Orphan crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
12 more citing papers are in PubMed but not listed here.
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Transgenic cassava (Manihot esculenta Crantz, cv MCol22) plants with a 92% reduction in cyanogenic glucoside content in tubers and acyanogenic (<1% of wild type) leaves were obtained by RNA interference to block expression of CYP79D1 and CYP79D2, the two paralogous genes encoding the first committed enzymes in linamarin and lotaustralin synthesis. About 180 independent lines with acyanogenic (<1% of wild type) leaves were obtained. Only a few of these were depleted with respect to cyanogenic glucoside content in tubers. In agreement with this observation, girdling experiments demonstrated that cyanogenic glucosides are synthesized in the shoot apex and transported to the root, resulting in a negative concentration gradient basipetal in the plant with the concentration of cyanogenic glucosides being highest in the shoot apex and the petiole of the first unfolded leaf. Supply of nitrogen increased the cyanogenic glucoside concentration in the shoot apex. In situ polymerase chain reaction studies demonstrated that CYP79D1 and CYP79D2 were preferentially expressed in leaf mesophyll cells positioned adjacent to the epidermis. In young petioles, preferential expression was observed in the epidermis, in the two first cortex cell layers, and in the endodermis together with pericycle cells and specific parenchymatic cells around the laticifers. These data demonstrate that it is possible to drastically reduce the linamarin and lotaustralin content in cassava tubers by blockage of cyanogenic glucoside synthesis in leaves and petioles. The reduced flux to the roots of reduced nitrogen in the form of cyanogenic glucosides did not prevent tuber formation.
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