ArticlePlant physiology2005
Mitochondria-driven changes in leaf NAD status exert a crucial influence on the control of nitrate assimilation and the integration of carbon and nitrogen metabolism.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73 citing papers in PubMed, 181 citations in OpenAlex.
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- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Distinct redox state regulation in the seedling performance of Norway maple and sycamore.Journal of plant research · 2023Article
- Mitochondrial Complex I Disruption Causes Broad Reorchestration of Plant Lipidome Including Chloroplast Lipids.International journal of molecular sciences · 2022Article
- Alterations in Primary Carbon Metabolism in Cucumber Infected withInternational journal of molecular sciences · 2022Article
- Quantitative Analysis of Redox Pool (NADBio-protocol · 2022Article
- Arabidopsis nitrate-induced aspartate oxidase gene expression is necessary to maintain metabolic balance under nitrogen nutrient fluctuation.Communications biology · 2022Article
- Photorespiration: The Futile Cycle?Plants (Basel, Switzerland) · 2021Review
- The Complete Mitogenome ofFrontiers in plant science · 2021Article
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- Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance.Scientific reports · 2019Article
- Deficiencies in the Mitochondrial Electron Transport Chain Affect Redox Poise and Resistance TowardFrontiers in plant science · 2019Article
- Regulation of Pyridine Nucleotide Metabolism During Tomato Fruit Development Through Transcript and Protein Profiling.Frontiers in plant science · 2019Article
- Physiological Significance of NAD Kinases in Cyanobacteria.Frontiers in plant science · 2019Review
- Nitrogen Source Dependent Changes in Central Sugar Metabolism Maintain Cell Wall Assembly in Mitochondrial Complex I-DefectiveInternational journal of molecular sciences · 2018Article
- Aldehyde Dehydrogenases Function in the Homeostasis of Pyridine Nucleotides in Arabidopsis thaliana.Scientific reports · 2018Article
- A lectin receptor kinase as a potential sensor for extracellular nicotinamide adenine dinucleotide ineLife · 2017Article
- Role of Type 2 NAD(P)H Dehydrogenase NdbC in Redox Regulation of Carbon Allocation inPlant physiology · 2017Article
- Transcriptome Analyses of Two Citrus Cultivars (Shiranuhi and Huangguogan) in Seedling Etiolation.Scientific reports · 2017Article
13 more citing papers are in PubMed but not listed here.
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6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Nicotiana sylvestris mutant, CMS, lacks the mitochondrial gene nad7 and functional complex I, and respires using low-affinity NADH (alternative) mitochondrial dehydrogenases. Here, we show that this adjustment of respiratory pathways is associated with a profound modification of foliar carbon-nitrogen balance. CMS leaves are characterized by abundant amino acids compared to either wild-type plants or CMS in which complex I function has been restored by nuclear transformation with the nad7 cDNA. The metabolite profile of CMS leaves is enriched in amino acids with low carbon/nitrogen and depleted in starch and 2-oxoglutarate. Deficiency in 2-oxoglutarate occurred despite increased citrate and malate and higher capacity of key anaplerotic enzymes, notably the mitochondrial NAD-dependent isocitrate dehydrogenase. The accumulation of nitrogen-rich amino acids was not accompanied by increased expression of enzymes involved in nitrogen assimilation. Partitioning of (15)N-nitrate into soluble amines was enhanced in CMS leaf discs compared to wild-type discs, especially in the dark. Analysis of pyridine nucleotides showed that both NAD and NADH were increased by 2-fold in CMS leaves. The growth retardation of CMS relative to the wild type was highly dependent on photoperiod, but at all photoperiod regimes the link between high contents of amino acids and NADH was observed. Together, the data provide strong evidence that (1) NADH availability is a critical factor in influencing the rate of nitrate assimilation and that (2) NAD status plays a crucial role in coordinating ammonia assimilation with the anaplerotic production of carbon skeletons.
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