ArticleAnnals of the rheumatic diseases2006
Analysis of RAG expression by peripheral blood CD5+ and CD5- B cells of patients with childhood systemic lupus erythematosus.
Article in Annals of the rheumatic diseases, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- BCR signal strength governs VH gene replacement in peripheral B cells.Cellular & molecular immunology · 2026Article
- The Role of B1 Cells in Systemic Lupus Erythematosus.Frontiers in immunology · 2022Review
- Structural gymnastics of RAG-mediated DNA cleavage in V(D)J recombination.Current opinion in structural biology · 2018Review
- Phosphorothioate-modified CpG oligodeoxynucleotides mimic autoantigens and reveal a potential role for Toll-like receptor 9 in receptor revision.Immunology · 2013Article
- A novel subpopulation of B-1 cells is enriched with autoreactivity in normal and lupus-prone mice.Arthritis and rheumatism · 2009Article
- B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice.The Journal of clinical investigation · 2008Article
- Interleukin-6 is responsible for aberrant B-cell receptor-mediated regulation of RAG expression in systemic lupus erythematosus.Immunology · 2007Article
- Differential expression patterns of recombination-activating genes in individual mature B cells in juvenile idiopathic arthritis.Annals of the rheumatic diseases · 2006Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe assembly of immunoglobulin genes during B cell development in the bone marrow is dependent on the expression of recombination activating genes (RAG) 1 and 2. Recently, RAG expression in peripheral blood IgD+ B cells outside the bone marrow has been demonstrated and is associated with the development of autoimmune diseases.
objectiveTo investigate RAG expression in the CD5+ or CD5- IgD+ B cell compartment in childhood systemic lupus erythematosus (SLE).
methodsUsing a combination of flow cytometric cell sorting and reverse transcriptase polymerase chain reaction analysis of cDNA libraries generated from individual cells, the expression of RAG, VpreB, and CD154 mRNA by individual peripheral blood B cells of three paediatric SLE patients was examined in detail.
resultsWhile only one patient had a significantly increased frequency of RAG+ B cells in the CD5- B cell population, all patients showed higher frequencies of RAG+ B cells in the CD5+IgD+ B cell population. The frequency of RAG+ IgD+CD5+/- B cells was reduced during intravenous cyclophosphamide treatment. In healthy age matched children, RAG expressing IgD+ B cells were hardly detectable. Coexpression of RAG and VpreB or CD154 mRNA could only be found in SLE B cells.
conclusionsRAG expression in peripheral blood B cells of SLE patients is particularly increased in the IgD+CD5+ B cell population. CD5+ and CD5- B cells in SLE have the potential to undergo receptor revision leading to the generation of high affinity pathogenic autoantibodies.
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