ArticleVirology journal2005
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to infectious salmon anaemia virus.
Article in Virology journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 68 citations in OpenAlex.
- ISGylation and E3 ubiquitin ligases: an Atlantic salmon genetic perspective.Frontiers in immunology · 2025Article
- Immune responses to Tilapia lake virus infection: what we know and what we don't know.Frontiers in immunology · 2023Review
- Exploring genetic resistance to infectious salmon anaemia virus in Atlantic salmon by genome-wide association and RNA sequencing.BMC genomics · 2021Article
- Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander,International journal of molecular sciences · 2020Article
- Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Ecology and evolution · 2019Article
- Using Gene Transcription to Assess Ecological and Anthropological Stressors in Brown Bears.EcoHealth · 2018Article
- Coupling gene-based and classic veterinary diagnostics improves interpretation of health and immune function in the Agassiz's desert tortoise (Conservation physiology · 2017Article
- Autochthonous Bacterial Isolates Successfully Stimulate In vitro Peripheral Blood Leukocytes of the European Sea Bass (Dicentrarchus labrax).Frontiers in microbiology · 2016Article
- Integrating Gene Transcription-Based Biomarkers to Understand Desert Tortoise and Ecosystem Health.EcoHealth · 2015Article
- Mx proteins: antiviral gatekeepers that restrain the uninvited.Microbiology and molecular biology reviews : MMBR · 2013Review
- Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus.Virology journal · 2010Article
- Infectious salmon anaemia virus replication and induction of alpha interferon in Atlantic salmon erythrocytes.Virology journal · 2008Article
- Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR.Journal of veterinary science · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious salmon anaemia (ISA) is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV), a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. Mx proteins are among the interferon (IFN)-induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction (RT-PCR) and Chinook salmon embryo (CHSE-214) cells constitutively expressing Atlantic salmon Mx1 protein (ASMx1) to examine the antiviral properties of ASMx1 against two ISAV strains, NBISA01 and HKS-36, having phenotypically different growth properties (cytopathic vs non-cytopathic) in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMx1. CHSE-214 cells constitutively expressing ASMx1 showed increased resistance to infection with the cytopathic ISAV strain NBISA01, manifested as delayed development of cytopathic effects (CPE) and significant reduction in the severity of CPE, as well as a 10-fold reduction in virus yield. However, by real-time RT-PCR we observed no significant difference in the mean threshold cycle (Ct) values of ISAV RNA levels, suggesting that the ASMx1 activity on ISAV occurs at the post-transcription steps of virus replication, possibly in the cytoplasm.
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