ArticleWorld journal of gastroenterology2005
IFN-gamma increases efficiency of DNA vaccine in protecting ducks against infection.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Efficiency of Interferon-γ in Activating Dendritic Cells and Its Potential Synergy with Toll-like Receptor Agonists.Viruses · 2023Article
- Article
- Technologies for enhanced efficacy of DNA vaccines.Expert review of vaccines · 2012Review
- Simple and rapid detection of human enterovirus 71 by reverse-transcription and loop-mediated isothermal amplification: cryopreservation affected the detection ability.Diagnostic microbiology and infectious disease · 2011Article
- Alginate-chitosan-PLGA composite microspheres enabling single-shot hepatitis B vaccination.The AAPS journal · 2010Article
- Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-gamma.Journal of veterinary science · 2009Article
- Contrasting evolution of diversity at two disease-associated chicken genes.Immunogenetics · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo detect the effects of DNA vaccines in combination with duck IFN-gamma gene on the protection of ducks against duck hepatitis B virus (DHBV) infection.
methodsDuIFN-gamma cDNA was cloned and expressed in COS-7 cells, and the antiviral activity of DuIFN-gamma was detected and neutralized by specific antibodies. Ducks were vaccinated with DHBpreS/S DNA alone or co-immunized with plasmid expressing DuIFN-gamma. DuIFN-gamma mRNA in peripheral blood mononuclear cells (PBMCs) from immunized ducks was detected by semi-quantitative competitive RT-PCR. Anti-DHBpreS was titrated by enzyme-linked immunosorbent assay (ELISA). DHBV DNA in sera and liver was detected by Southern blot hybridization, after ducks were challenged with high doses of DHBV.
resultsDuIFN-gamma expressed by COS-7 was able to protect duck fibroblasts against vesicular stomatitis virus (VSV) infection in a dose-dependent fashion, and anti-DuIFN-gamma antibodies neutralized the antiviral effects. DuIFN-gamma in the supernatant also inhibited the release of DHBV DNA from LMH-D2 cells. When ducks were co-immunized with DNA vaccine expressing DHBpreS/S and DuIFN-gamma gene as an adjuvant, the level of DuIFN-gamma mRNA in PBMCs was higher than that in ducks vaccinated with DHBpreS/S DNA alone. However, the titer of anti-DHBpreS elicited by DHBpreS/S DNA alone was higher than that co-immunized with DuIFN-gamma gene and DHBpreS/S DNA. After being challenged with DHBV at high doses, the load of DHBV in sera dropped faster, and the amount of total DNA and cccDNA in the liver decreased more significantly in the group of ducks co-immunized with DuIFN-gamma gene and DHBpreS/S DNA than in other groups.
conclusionDHBV preS/S DNA vaccine can protect ducks against DHBV infection, DuIFN-gamma gene as an immune adjuvant enhances its efficacy.
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