ArticleProceedings of the National Academy of Sciences of the United States of America2005
A third Na+-binding site in the sodium pump.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 61 citations in OpenAlex.
- Structure and function of HNature communications · 2022Article
- Disease mutations of human α3 NaPNAS nexus · 2022Article
- Na/K-ATPase beta1-subunit associates with neuronal growth regulator 1 (NEGR1) to participate in intercellular interactions.BMB reports · 2021Article
- Distinct effects of Q925 mutation on intracellular and extracellular NaScientific reports · 2019Article
- Functional consequences of the CAPOS mutation E818K of NaThe Journal of biological chemistry · 2019Article
- Targeted Molecular Characterization of Aldosterone-Producing Adenomas in White Americans.The Journal of clinical endocrinology and metabolism · 2018Article
- On the effect of hyperaldosteronism-inducing mutations in Na/K pumps.The Journal of general physiology · 2017Article
- Arginine substitution of a cysteine in transmembrane helix M8 converts Na+,K+-ATPase to an electroneutral pump similar to H+,K+-ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Molecular Mechanisms and Kinetic Effects of FXYD1 and Phosphomimetic Mutants on Purified Human Na,K-ATPase.The Journal of biological chemistry · 2015Article
- Importance of the Voltage Dependence of Cardiac Na/K ATPase Isozymes.Biophysical journal · 2015Article
- Route, mechanism, and implications of proton import during Na+/K+ exchange by native Na+/K+-ATPase pumps.The Journal of general physiology · 2014Article
- Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension.Nature genetics · 2013Article
- Article
- Branchial Na(+):K(+):2Cl(-) cotransporter 1 and Na(+)/K(+)-ATPase α-subunit in a brackish water-type ionocyte of the euryhaline freshwater white-rimmed stingray, Himantura signifer.Frontiers in physiology · 2013Article
- Effects of oligomycin on transient currents carried by Na+ translocation of Bufo Na+/K(+)-ATPase expressed in Xenopus oocytes.The Journal of membrane biology · 2011Article
- FXYD proteins stabilize Na,K-ATPase: amplification of specific phosphatidylserine-protein interactions.The Journal of biological chemistry · 2011Article
- A structural overview of the plasma membrane Na+,K+-ATPase and H+-ATPase ion pumps.Nature reviews. Molecular cell biology · 2011Review
- Selectivity of externally facing ion-binding sites in the Na/K pump to alkali metals and organic cations.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The rapid-onset dystonia parkinsonism mutation D923N of the Na+, K+-ATPase alpha3 isoform disrupts Na+ interaction at the third Na+ site.The Journal of biological chemistry · 2010Article
- The two C-terminal tyrosines stabilize occluded Na/K pump conformations containing Na or K ions.The Journal of general physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sodium pump, or Na,K-ATPase, exports three intracellular sodium ions in exchange for two extracellular potassium ions. In the high resolution structure of the related calcium pump, two cation-binding sites have been identified. The two corresponding sites in the sodium pump are expected to be alternatively occupied by sodium and potassium. The position of a third sodium-specific site is still hypothetical. Here, we report the large effects of single residue substitutions on the voltage-dependent kinetics of the release of sodium to the extracellular side of the membrane. These mutations also alter the apparent affinity for intracellular sodium while one of them does not affect the intrinsic affinity for potassium. These results enable us to locate the third sodium-specific site of the sodium pump in a space between the fifth, sixth, and ninth transmembrane helices of the alpha-subunit and provide an experimental validation of the model proposed by Ogawa and Toyoshima [Ogawa, H. & Toyoshima, C. (2002) Proc. Natl. Acad. Sci. USA 99, 15977-15982].
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.