ArticleMolecular biology of the cell2005
Myosin-V, Kinesin-1, and Kinesin-3 cooperate in hyphal growth of the fungus Ustilago maydis.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 114 citations in OpenAlex.
- Kinesin-1 autoinhibition facilitates the initiation of dynein cargo transport.The Journal of cell biology · 2023Article
- The type V myosin-containing complex HUM is a RAB11 effector powering movement of secretory vesicles.iScience · 2022Article
- A Kinesin Vdkin2 Required for Vacuole Formation, Mycelium Growth, and Penetration Structure Formation ofJournal of fungi (Basel, Switzerland) · 2022Article
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Investigating the Smuts: Common Cues, Signaling Pathways, and the Role ofJournal of fungi (Basel, Switzerland) · 2020Review
- Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes.Microorganisms · 2020Review
- Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis.Scientific reports · 2018Article
- A Botrytis cinerea KLP-7 Kinesin acts as a Virulence Determinant during Plant Infection.Scientific reports · 2017Article
- BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional Movement of Lysosomes along Different Microtubule Tracks.Cell reports · 2016Article
- Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.Nature communications · 2016Article
- Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Selection of reference genes for quantitative real time RT-PCR during dimorphism in the zygomycete Mucor circinelloides.Molecular biology reports · 2015Article
- Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin.PloS one · 2015Article
- A method for multiprotein assembly in cells reveals independent action of kinesins in complex.The Journal of cell biology · 2014Article
- Long-distance endosome trafficking drives fungal effector production during plant infection.Nature communications · 2014Article
- The Vip1 inositol polyphosphate kinase family regulates polarized growth and modulates the microtubule cytoskeleton in fungi.PLoS genetics · 2014Article
- Endocytosis and early endosome motility in filamentous fungi.Current opinion in microbiology · 2014Review
- Kinesins have a dual function in organizing microtubules during both tip growth and cytokinesis in Physcomitrella patens.The Plant cell · 2014Article
- The SPF27 homologue Num1 connects splicing and kinesin 1-dependent cytoplasmic trafficking in Ustilago maydis.PLoS genetics · 2014Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-distance transport is crucial for polar-growing cells, such as neurons and fungal hyphae. Kinesins and myosins participate in this process, but their functional interplay is poorly understood. Here, we investigate the role of kinesin motors in hyphal growth of the plant pathogen Ustilago maydis. Although the microtubule plus-ends are directed to the hyphal tip, of all 10 kinesins analyzed, only conventional kinesin (Kinesin-1) and Unc104/Kif1A-like kinesin (Kinesin-3) were up-regulated in hyphae and they are essential for extended hyphal growth. deltakin1 and deltakin3 mutant hyphae grew irregular and remained short, but they were still able to grow polarized. No additional phenotype was detected in deltakin1rkin3 double mutants, but polarity was lost in deltamyo5rkin1 and deltamyo5rkin3 mutant cells, suggesting that kinesins and class V myosin cooperate in hyphal growth. Consistent with such a role in secretion, fusion proteins of green fluorescent protein and Kinesin-1, Myosin-V, and Kinesin-3 accumulate in the apex of hyphae, a region where secretory vesicles cluster to form the fungal Spitzenkörper. Quantitative assays revealed a role of Kin3 in secretion of acid phosphatase, whereas Kin1 was not involved. Our data demonstrate that just two kinesins and at least one myosin support hyphal growth.
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