ArticleGenetics2006
Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes.Life science alliance · 2022Article
- The Srs2 helicase dampens DNA damage checkpoint by recycling RPA from chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phospho-Regulation of Meiotic Prophase.Frontiers in cell and developmental biology · 2021Review
- ATR is required to complete meiotic recombination in mice.Nature communications · 2018Article
- Differential RPA-1 and RAD-51 recruitment in vivo throughout the C. elegans germline, as revealed by laser microirradiation.Nucleic acids research · 2018Article
- Profiling DNA damage-induced phosphorylation in budding yeast reveals diverse signaling networks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- RPA homologs and ssDNA processing during meiotic recombination.Chromosoma · 2016Review
- Meiotic Recombination: The Essence of Heredity.Cold Spring Harbor perspectives in biology · 2015Review
- Rice MEL2, the RNA recognition motif (RRM) protein, binds in vitro to meiosis-expressed genes containing U-rich RNA consensus sequences in the 3'-UTR.Plant molecular biology · 2015Article
- Article
- Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae.Experimental cell research · 2015Article
- The meiotic checkpoint network: step-by-step through meiotic prophase.Cold Spring Harbor perspectives in biology · 2014Review
- The recombinases DMC1 and RAD51 are functionally and spatially separated during meiosis in Arabidopsis.The Plant cell · 2012Article
- Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae.DNA repair · 2009Review
- Regulatory functions of the N-terminal domain of the 70-kDa subunit of replication protein A (RPA).The Journal of biological chemistry · 2008Article
- ATM promotes the obligate XY crossover and both crossover control and chromosome axis integrity on autosomes.PLoS genetics · 2008Article
- Meiotic roles of Mec1, a budding yeast homolog of mammalian ATR/ATM.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Replication protein A (RPA) is the major single-stranded DNA-binding protein in eukaryotes, essential for DNA replication, repair, and recombination. During mitosis and meiosis in budding yeast, RPA becomes phosphorylated in reactions that require the Mec1 protein kinase, a central checkpoint regulator and homolog of human ATR. Through mass spectrometry and site-directed mutagenesis, we have now identified a single serine residue in the middle subunit of the RPA heterotrimer that is targeted for phosphorylation by Mec1 both in vivo and in vitro. Cells containing a phosphomimetic version of RPA generated by mutation of this serine to aspartate exhibit a significant alteration in the pattern of meiotic crossovers for specific genetic intervals. These results suggest a new function of Mec1 that operates through RPA to locally control reciprocal recombination.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.