ArticleBMC cancer2005
Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis.
Article in BMC cancer, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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40 citing papers in PubMed, 1 synthesis or guideline pooled it, 173 citations in OpenAlex.
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- Identification of potential serum biomarkers for Wilms tumor after excluding confounding effects of common systemic inflammatory factors.Molecular biology reports · 2012Article
- Serum amyloid A: A new potential serum marker correlated with the stage of breast cancer.Oncology letters · 2012Article
- Purification, identification and profiling of serum amyloid A proteins from sera of advanced-stage cancer patients.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2012Article
- Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries.Molecular & cellular proteomics : MCP · 2011Article
- Improving Detection Accuracy of Lung Cancer Serum Proteomic Profiling via Two-Stage Training Process.Proteome science · 2011Article
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Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCancer serum protein profiling by mass spectrometry has uncovered mass profiles that are potentially diagnostic for several common types of cancer. However, direct mass spectrometric profiling has a limited dynamic range and difficulties in providing the identification of the distinctive proteins. We hypothesized that distinctive profiles may result from the differential expression of relatively abundant serum proteins associated with the host response.
methodsEighty-four antibodies, targeting a wide range of serum proteins, were spotted onto nitrocellulose-coated microscope slides. The abundances of the corresponding proteins were measured in 80 serum samples, from 24 newly diagnosed subjects with lung cancer, 24 healthy controls, and 32 subjects with chronic obstructive pulmonary disease (COPD). Two-color rolling-circle amplification was used to measure protein abundance.
resultsSeven of the 84 antibodies gave a significant difference (p < 0.01) for the lung cancer patients as compared to healthy controls, as well as compared to COPD patients. Proteins that exhibited higher abundances in the lung cancer samples relative to the control samples included C-reactive protein (CRP; a 13.3 fold increase), serum amyloid A (SAA; a 2.0 fold increase), mucin 1 and alpha-1-antitrypsin (1.4 fold increases). The increased expression levels of CRP and SAA were validated by Western blot analysis. Leave-one-out cross-validation was used to construct Diagonal Linear Discriminant Analysis (DLDA) classifiers. At a cutoff where all 56 of the non-tumor samples were correctly classified, 15/24 lung tumor patient sera were correctly classified.
conclusionOur results suggest that a distinctive serum protein profile involving abundant proteins may be observed in lung cancer patients relative to healthy subjects or patients with chronic disease and may have utility as part of strategies for detecting lung cancer.
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